ArticleBMC veterinary research2025
Development of an in-house indirect ELISA kit for the serological detection of antibodies against infectious bursal disease in chickens.
Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.
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Who cites it
3 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Prevalence of infectious bursal disease in chicken populations in China during 1983-2024: a meta-analysis.Frontiers in veterinary science · 2026Pooled it
- Development and Validation of an In-House Indirect ELISA for Serological Detection ofVeterinary medicine international · 2026Article
- Development and Application of Indirect ELISA for IBDV VP2 Antibodies Detection in Poultry.Viruses · 2025Article
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Authors and funding
20 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infectious bursal disease is a highly contagious disease of young chickens caused by the infectious bursal disease virus. This disease poses an important threat to the commercial poultry industry globally. This study was designed to develop an In-House Indirect Enzyme-Linked Immune Sorbent Assay Kit for the serological detection of antibodies against infectious bursal disease viruses. An infectious bursal disease virus antigen dilution (1:2), sample serum (1:500), and mouse anti-chicken immunoglobulin G (IgG) labeled with horseradish peroxidase (HRP) (1:2,000) were used in this assay. The calculated cutoff value was 0.24. This in-house indirect ELISA method was compared with a commercial ELISA kit for the detection of antibodies against infectious bursal disease virus in chickens. The performance of the newly developed and commercial ELISA kit was evaluated as described by Samad et al. (1994). The sensitivity and specificity of the current ELISA method were 98% (95% CI: 92.96-99.76) and 97% (95% CI: 91.48-99.38), respectively. The average intra-assay % CV of the triplet of 2 samples was 7.6, and interassay comparisons indicated a CV of 5.45%. As indicated by the results, we described a valuable and cost-effective, sensitive and specific in-house indirect ELISA kit for the serological diagnosis of infectious bursal disease in Ethiopia.
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