Evidence map›Paper›PMID 40287078›Full record

ArticleInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases2025

Plasmodium falciparum genetic diversity and multiplicity of infection in northern and southern Ghana assessed by amplicon sequencing.

Xiaoyun Wei, Awtum Brashear, Faiza Siddiqui, Georgina Agyekum, Amuza Lucky, Anongruk Chim-Ong, Yaw Afrane, Jun Miao, Chengqi Wang, Linda Amoah and 1 more

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Article in Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Genetic Diversity and Allelic Distribution ofJournal of parasitology research · 2026
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xiaoyun WeiDepartment of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.
Awtum BrashearDepartment of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.
Faiza SiddiquiDepartment of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.
Georgina AgyekumDepartment of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.
Amuza LuckyDepartment of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.
Anongruk Chim-OngDepartment of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.
Yaw AfraneDepartment of Medical Microbiology, University of Ghana Medical School, University of Ghana, Korle-Bu, Accra, Ghana.
Jun MiaoDepartment of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.
Chengqi WangDepartment of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.
Linda AmoahDepartment of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana. Electronic address: levaamoah@noguchi.ug.edu.gh.
Liwang CuiDepartment of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA. Electronic address: liwangcui@usf.edu.

Funding

Unraveling the Drivers of Persistent Malaria Transmission in Cameroon: A Systems Approach (REDIAL)U19AI181593 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI LIWANG CUI · 2024 to 2026
$3.0M
NIAID NIH HHS U19 AI181593
6 · The paper itself

Abstract

Malaria is a significant public health challenge in Ghana, with Plasmodium falciparum accounting for 80-90 % of infections. This study aimed to determine whether different climatic conditions, demographics, transmission patterns, and control practices in northern and southern Ghana result in differences in the genetic diversity, population structure, and evolutionary dynamics of P. falciparum. A total of 354 clinical samples collected in 2018 and 2021 were analyzed using the amplicon sequencing approach, targeting the CSP, AMA1, SERA2, and TRAP genes. Parasite populations from both northern and southern Ghana exhibited high haplotype diversity (Hd = 0.93-0.98). More than 70 % of the patient samples contained multiclonal infections, and the multiplicity of infection was significantly higher in northern (2.91) than in southern Ghana (2.42). Despite such a difference, extensive haplotype sharing was observed. The haplotype network and phylogenetic analyses highlighted substantial genetic connectivity among the parasite populations, likely driven by multiple factors, including human movement and shared vectors. Predominant haplotypes were consistent across regions and years, reflecting a stable pool of core haplotypes, which was not significantly impacted by contemporary control measures. In conclusion, this study underscored the high genetic diversity and connectivity of P. falciparum populations in Ghana despite different transmission ecology and the implementation of divergent control measures.

Indexed as

DNA, ProtozoanGene AmplificationGenetic VariationMalaria, FalciparumPlasmodium falciparumDNA Sequence, UnstableGhanaHaplotypesHumansDNA, ProtozoanAmplicon sequencingGenetic diversityMultiplicity of infectionPlasmodium falciparumPopulation structure

Identifiers

PMID40287078
PMCPMC12520160

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.