Evidence map›Paper›PMID 40286162›Full record

ArticleMolecules (Basel, Switzerland)2025

Cholinium-Based Ionic Liquids Modulate Protein Stability: A Comparative Study of Enzymes and Albumins.

Artashes A Khachatrian, Timur A Mukhametzyanov, Ramazan Z Salikhov, Alexandra E Klimova, Dmitry G Yakhvarov, Bulat F Garifullin, Olga S Terenteva, Pavel L Padnya, Ivan I Stoikov, Boris N Solomonov

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Artashes A KhachatrianDepartment of Physical Chemistry, Kazan Federal University, Kremlyovskaya 18, Kazan 420008, Russia.
Timur A MukhametzyanovDepartment of Physical Chemistry, Kazan Federal University, Kremlyovskaya 18, Kazan 420008, Russia.ORCID 0000-0003-2645-5110
Ramazan Z SalikhovDepartment of Physical Chemistry, Kazan Federal University, Kremlyovskaya 18, Kazan 420008, Russia.
Alexandra E KlimovaDepartment of Physical Chemistry, Kazan Federal University, Kremlyovskaya 18, Kazan 420008, Russia.ORCID 0009-0002-2931-5957
Dmitry G YakhvarovDepartment of Physical Chemistry, Kazan Federal University, Kremlyovskaya 18, Kazan 420008, Russia.ORCID 0000-0002-3906-8841
Bulat F GarifullinArbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, Arbuzov St. 8, Kazan 420008, Russia.ORCID 0000-0002-7590-3832
Olga S TerentevaDepartment of Organic and Medicinal Chemistry, Kazan Federal University, Kremlyovskaya 18, Kazan 420008, Russia.ORCID 0000-0002-4609-057X
Pavel L PadnyaDepartment of Organic and Medicinal Chemistry, Kazan Federal University, Kremlyovskaya 18, Kazan 420008, Russia.ORCID 0000-0002-1924-8028
Ivan I StoikovDepartment of Organic and Medicinal Chemistry, Kazan Federal University, Kremlyovskaya 18, Kazan 420008, Russia.ORCID 0000-0002-3019-7866
Boris N SolomonovDepartment of Physical Chemistry, Kazan Federal University, Kremlyovskaya 18, Kazan 420008, Russia.

Funding

Russian Science Foundation Project No 24-73-00164 https://rscf.ru/project/24-73-00164
6 · The paper itself

Abstract

This work aims to assess the intermolecular interaction of choline ionic liquids (ILs) (choline malonate ([Ch][Mal]), choline succinate ([Ch][Suc]), and choline valinate ([Ch][Val]) with two enzymes (lysozyme and α-chymotrypsin). We evaluated the state of the tertiary protein structure using circular dichroism (CD) spectrometry and quantified the binding parameters of the binding of the ionic liquids to the enzymes by fluorescence spectroscopy. The binding energies of the enzymes and the localization of ions on them were estimated using the molecular docking. We then analyzed the relationship between the enzymes' thermostability and their tendency towards aggregation in the enzyme/ionic liquid systems. The obtained results were compared with previous data on albumins to identify similarities and differences between the behavior of enzymes and albumins in ionic liquid solutions. Despite the comparable values of the binding constants, the effect of ionic liquids on the thermostability of enzymes was the opposite of their effect on albumins. In addition, although these ionic liquids promoted aggregation in both enzymes and albumins, this effect was much more pronounced for albumins.

Indexed as

AlbuminsCholineChymotrypsinIonic LiquidsMuramidaseCircular DichroismEnzyme StabilityMolecular Docking SimulationProtein BindingProtein StabilitySpectrometry, FluorescenceAlbuminsalpha-chymotrypsinCholineChymotrypsinIonic LiquidsMuramidasebindingenzymeionic liquidproteinstructure

Identifiers

PMID40286162
PMCPMC11990322

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.