Analysis of ligand binding mechanism by dimeric receptors using stopped-flow fluorimetry-application to the human decapping scavenger enzyme.

Zbigniew M Darzynkiewicz, Megerditch Kiledjian, Jan M Antosiewicz

Abstract read

In one paragraph

Article in European biophysics journal : EBJ, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Zbigniew M DarzynkiewiczDivision of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Pasteura 5, 02-093, Warsaw, Poland. darzynkiewicz@gmail.com.

Megerditch KiledjianDepartment of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey, 08854-8082, USA. kiledjian@biology.rutgers.edu.

Jan M AntosiewiczDivision of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Pasteura 5, 02-093, Warsaw, Poland. jantosi@fuw.edu.pl.

Funding

5’ end RNA Caps in Gene ExpressionR35GM149262 · NIGMS · RUTGERS, THE STATE UNIV OF N.J. · PI MEGERDITCH KILEDJIAN · 2023 to 2026

$2.3M

Foundation for the National Institutes of Health GM835487Narodowe Centrum Nauki UMO-2013/08/A/NZ1/00866Narodowe Centrum Nauki UMO-2014/13/B/ST4/03011NIGMS NIH HHS R35 GM149262

6 · The paper itself

Abstract

Association of a ligand with the binding site of a receptor is usually at least a two-step process - formation of an initial encounter complex followed by a conformational transition of the complex. Consequently, the description of binding by dimeric receptors requires a two-dimensional reaction scheme. An interesting example of a dimeric receptor is the decapping scavenger enzyme, DcpS. It is a critical determinant of mRNA metabolism that hydrolyses the 5'-end

Indexed as

EndoribonucleasesFluorometryProtein MultimerizationBinding SitesCatalytic DomainDimerizationHumansKineticsLigandsProtein BindingRNA CapsDcpS protein, humanEndoribonucleasesLigandsRNA CapsHomo-dimer receptorKineticsLigand bindingmRNA degradationNumerical analysisProgress curves

Identifiers

PMID40285819

DOI10.1007/s00249-025-01748-w

PMCPMC13110873

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.