Evidence map›Paper›PMID 40285610›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Extracellular Vesicular Delta-Like Ligand 3 and Subtype Transcription Factors for Small Cell Lung Cancer Diagnosis.

Hong Li, Chi-Ling Chiang, Kwang Joo Kwak, Hsin-Lun Lee, Xinyu Wang, Giulia Romano, Michela Saviana, Robin Toft, Tai-Shan Cheng, Yuehshih Chang and 18 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors.

Hong LiDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
Chi-Ling ChiangDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
Kwang Joo KwakSpot Biosystems Ltd., Columbus, OH, 43212, USA.
Hsin-Lun LeeDepartment of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.
Xinyu WangDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
Giulia RomanoDivision of Pulmonary Diseases and Critical Care Medicine, Virginia Commonwealth University, Richmond, VA, 23284, USA.
Michela SavianaDivision of Pulmonary Diseases and Critical Care Medicine, Virginia Commonwealth University, Richmond, VA, 23284, USA.
Robin ToftDivision of Pulmonary Diseases and Critical Care Medicine, Virginia Commonwealth University, Richmond, VA, 23284, USA.
Tai-Shan ChengInstitute of Biopharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
Yuehshih ChangDivision of Hematology and Oncology, Department of Internal Medicine, Keelung Chang Gung Memorial Hospital, Keelung, 20401, Taiwan.
Bi-Da HsiangInstitute of Biopharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
Guan-Wan LiuInstitute of Biopharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
Xiaokui MoCenter for Biostatistics, The Ohio State University, Columbus, OH, 43210, USA.
Yifan MaDepartment of Biomedical Engineering, The Ohio State University, Columbus, OH, 43210, USA.
Junjie PanDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
Xilal Y RimaDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
Truc Nguyen KimDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
Eduardo ReateguiDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.
Chia-Ning ShenGenomic Research Center, Academia Sinica, Taipei, 11529, Taiwan.
Yeh-Shiu ChuBrain Research Center, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
Carlo CroceCollege of Medicine, The Ohio State University, Columbus, OH, 43210, USA.
Peter Mu-Hsin ChangInstitute of Biopharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
Yi-Chen YehSchool of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
David P CarboneCollege of Medicine, The Ohio State University, Columbus, OH, 43210, USA.
Chi-Ying F HuangInstitute of Biopharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
Chi-Lu ChiangSchool of Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
Patrick Nana-SinkamDivision of Pulmonary Diseases and Critical Care Medicine, Virginia Commonwealth University, Richmond, VA, 23284, USA.
L James LeeDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.ORCID https://orcid.org/0000-0002-3589-0280

Funding

Extracellular Vesicles in Small Cell Lung Cancer Early DetectionU01CA213330 · NCI · VIRGINIA COMMONWEALTH UNIVERSITY · PI LEE, LY JAMES, NANASINKAM, SERGE PATRICK · 2017 to 2021
$2.7M
Multi-parametric Integrated Molecular Detection of SARS-CoV-2 from Biofluids by Adapting Single Extracellular Vesicle Characterization TechnologiesU18TR003807 · NCATS · OHIO STATE UNIVERSITY · PI LEE, INYOUL, LEE, LY JAMES · 2021 to 2022
$1.8M
Microfluidics Array Based Sorting, Isolation, and RNA Analysis in Single Extracellular V csiclesUH3TR002884 · NCATS · OHIO STATE UNIVERSITY · PI KIM, BETTY, REATEGUI, EDUARDO · 2021 to 2022
$1.7M
Microfluidics Array Based Sorting, Isolation, and RNA Analysis in Single Extracellular V csiclesUG3TR002884 · NCATS · OHIO STATE UNIVERSITY · PI KIM, BETTY, REATEGUI, EDUARDO · 2019 to 2020
$822k
Chong Hin Loon Memorial Cancer and Biotherapy Research Center, National Yang-Ming UniversityMelissa Lee Cancer Foundation MLCF_V112_B11205Melissa Lee Cancer Foundation MLCF_V113_A11302Ministry of Education 113W031101Ministry of Science and Technology 108-2314-B-038-064National Science and Technology Council MOST108-2314-B-075-029National Science and Technology Council NSTC113-2314-B-A49-029-MY3NCATS NIH HHS U18 TR003807NCATS NIH HHS UG3 TR002884NCATS NIH HHS UH3 TR002884NCI NIH HHS U01 CA213330Taipei Veterans General Hospital V112C-004Taipei Veterans General Hospital V113C-040Taipei Veterans General Hospital V114C-207Yushan Fellow Program by the Ministry of Education
6 · The paper itself

Abstract

Small cell lung cancer (SCLC) is associated with high mortality and limited therapeutic options. There is increasing recognition that SCLC harbors molecular heterogeneity. Using a new liquid biopsy assay, it is demonstrated that SCLC subtypes, as determined by patient tumor tissue staining and cell lines, can be accurately identified by measuring the mRNA expression of subtype transcription factors (ASCL1, POU2F3, and NEUROD1) in circulating exosome-rich extracellular vesicles (Exo). Additionally, upregulation of Delta-like ligand 3 (DLL3) mRNA in Exo and its membrane protein (mProtein) in extracellular vesicles associated with tumor (tEV) may distinguish both limited- and extensive-stage SCLC patients from high-risk smokers, with AUC/ROC values of 0.836 and 0.839, respectively. By incorporating Exo-ASCL1 and Exo-POU2F3 mRNA expression with DLL3 Exo-mRNA/tEV-mProtein expression, the classifier enhances the AUC/ROC to 0.912 and 0.963 for limited- and extensive-stage SCLC patients, respectively.

Indexed as

Intracellular Signaling Peptides and ProteinsLung NeoplasmsMembrane ProteinsSmall Cell Lung CarcinomaTranscription FactorsBiomarkers, TumorExtracellular VesiclesFemaleHumansMaleBiomarkers, TumorDLL3 protein, humanIntracellular Signaling Peptides and ProteinsMembrane ProteinsTranscription FactorsDLL3 mRNA in exosomes and protein in tumor‐associated extracellular vesiclesimmune lipoplex nanoparticle biochip assayPOU2F3 and ASCL1 mRNA in exosomesSCLC detectionsingle extracellular vesicle analysis

Identifiers

PMID40285610
PMCPMC12165112

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.