Evidence map›Paper›PMID 40285608›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

In Vivo Cytosolic Delivery of Biomolecules into Neurons for Super-Resolution Imaging and Genome Modification.

Xiaoqian Ge, Joseph B Wekselblatt, Scott Elmore, Bo Wang, Tongtong Wang, Renjinming Dai, Tingting Zhang, Harsh Dave, Mohammadaref Ghaderi, Athul Raj Anilkumar and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Xiaoqian GeDepartment of Biomedical Engineering, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
Joseph B WekselblattDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Scott ElmoreDepartment of Chemistry and Biochemistry, University of Texas at Dallas, Richardson, TX, 75080-3021, USA.
Bo WangDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Tongtong WangDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Renjinming DaiDepartment of Bioengineering, University of Texas at Dallas, Richardson, TX, 75080-3021, USA.
Tingting ZhangDepartment of Mechanical Engineering, University of Texas at Dallas, Richardson, TX, 75080-3021, USA.
Harsh DaveDepartment of Bioengineering, University of Texas at Dallas, Richardson, TX, 75080-3021, USA.
Mohammadaref GhaderiDepartment of Bioengineering, University of Texas at Dallas, Richardson, TX, 75080-3021, USA.
Athul Raj AnilkumarDepartment of Bioengineering, University of Texas at Dallas, Richardson, TX, 75080-3021, USA.
Bill WangDepartment of Bioengineering, University of Texas at Dallas, Richardson, TX, 75080-3021, USA.
Shashank R SirsiDepartment of Bioengineering, University of Texas at Dallas, Richardson, TX, 75080-3021, USA.
Jung-Mo AhnDepartment of Chemistry and Biochemistry, University of Texas at Dallas, Richardson, TX, 75080-3021, USA.
Mikhail G ShapiroDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Yuki OkaDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Carlos LoisDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Zhenpeng QinDepartment of Biomedical Engineering, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.ORCID https://orcid.org/0000-0003-3406-3045

Funding

Development of new photo-releasable neuropeptide nano-vesicles for studying modulation in the brainRF1NS110499 · NINDS · UNIVERSITY OF TEXAS DALLAS · PI QIN, ZHENPENG, SLESINGER, PAUL A · 2019 to 2019
$2.4M
Enhancing Non-Viral Neuron-Specific Molecule Delivery Across Species in the CNSR01MH140482 · NIMH · UNIVERSITY OF TEXAS DALLAS · PI Yuki Oka, Zhenpeng Qin · 2025 to 2026
$1.5M
American Heart Association 19CSLOI34770004Cancer Prevention and Research Institute of Texas RP190278Cancer Prevention and Research Institute of Texas RP210236Department of Defense W81XWH-21-1-0219National Science Foundation 2123971NIH HHS R01MH140482NIH HHS RF1NS110499NIMH NIH HHS R01 MH140482NINDS NIH HHS RF1 NS110499
6 · The paper itself

Abstract

Efficient delivery of biomolecules into neurons has significant impacts on therapeutic applications in the central nervous system (CNS) and fundamental neuroscience research. Existing viral and non-viral delivery methods often suffer from inefficient intracellular access due to the endocytic pathway. Here, a neuron-targeting and direct cytosolic delivery platform is discovered by using a 15-amino-acid peptide, termed the N1 peptide, which enables neuron-specific targeting and cytosolic delivery of functional biomolecules. The N1 peptide initially binds hyaluronan in the extracellular matrix and subsequently passes the membrane of neurons without being trapped into endosome. This mechanism facilitates the efficient delivery of cell-impermeable and photo-stable fluorescent dye for super-resolution imaging of dendritic spines, and functional proteins, such as Cre recombinase, for site-specific genome modification. Importantly, the N1 peptide exhibits robust neuronal specificity across diverse species, including mice, rats, tree shrews, and zebra finches. Its targeting capability is further demonstrated through various administration routes, including intraparenchymal, intrathecal, and intravenous (i.v.) injections after blood-brain barrier (BBB) opening with focused ultrasound (FUS). These findings establish the N1 peptide as a versatile and functional platform with significant potential for bioimaging and advanced therapeutic applications.

Indexed as

CytosolDrug Delivery SystemsNeuronsAnimalsBlood-Brain BarrierMiceRatsacross speciesgenome modificationneuron specific targetingpeptidessuper‐resolution imaging

Identifiers

PMID40285608
PMCPMC12224921

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.