Evidence map›Paper›PMID 40285580›Full record

ArticleAdvanced materials (Deerfield Beach, Fla.)2025

Bioxolography Using Diphenyliodonium Chloride and N-Vinylpyrrolidone Enables Rapid High-Resolution Volumetric 3D Printing of Spatially Encoded Living Matter.

Alexis Wolfel, Castro Johnbosco, Annalise Anspach, Marieke Meteling, Jos Olijve, Niklas Felix König, Jeroen Leijten

Abstract read
In one paragraph

Article in Advanced materials (Deerfield Beach, Fla.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Alexis WolfelLeijten Lab, BioEngineering Technologies, TechMed Centre, Faculty of Science and Technology, University of Twente, Enschede, 7522NB, The Netherlands.ORCID https://orcid.org/0000-0002-4577-1699
Castro JohnboscoLeijten Lab, BioEngineering Technologies, TechMed Centre, Faculty of Science and Technology, University of Twente, Enschede, 7522NB, The Netherlands.ORCID https://orcid.org/0000-0003-3617-4603
Annalise AnspachLeijten Lab, BioEngineering Technologies, TechMed Centre, Faculty of Science and Technology, University of Twente, Enschede, 7522NB, The Netherlands.
Marieke MetelingLeijten Lab, BioEngineering Technologies, TechMed Centre, Faculty of Science and Technology, University of Twente, Enschede, 7522NB, The Netherlands.ORCID https://orcid.org/0009-0006-3562-7921
Jos OlijveRousselot BV, Port Arthurlaan 173, Ghent, 9000, Belgium.
Niklas Felix Königxolo GmbH, Volmerstraße 9B, 12489, Berlin, Germany.ORCID https://orcid.org/0000-0002-9293-3734
Jeroen LeijtenLeijten Lab, BioEngineering Technologies, TechMed Centre, Faculty of Science and Technology, University of Twente, Enschede, 7522NB, The Netherlands.ORCID https://orcid.org/0000-0002-8063-207X

Funding

HORIZON EUROPE European Innovation Council ELM-210803707Nederlandse Organisatie voor Wetenschappelijk Onderzoek NWO-XS grant 16998University of Twente TKI-HTSM-CALL12
6 · The paper itself

Abstract

Light-based volumetric bioprinting enables fabrication of cubic centimeter-sized living materials with micrometer resolution in minutes. Xolography is a light sheet-based volumetric printing technology that offers unprecedented volumetric generation rates and print resolutions for hard plastics. However, the limited solubility and reactivity of current dual-color photoinitiators (DCPIs) in aqueous media have hindered their application for high-resolution bioprinting of living matter. Here, we present a novel three-component formulation that drastically improves photoreactivity and thereby enables high-resolution, rapid, and cytocompatible Xolographic biofabrication of intricately architected yet mechanically robust living materials. To achieve this, various relevant additives are systematically explored, which revealed that diphenyliodonium chloride and N-vinylpyrrolidone strongly enhance D-mediated photoreactivity, as confirmed by dual-color photo-rheology. This enables Xolographic bioprinting of gelatin methacryloyl-based bioresins, producing >1 cm

Indexed as

BioprintingOnium CompoundsPrinting, Three-DimensionalPyrrolidinonesAnimalsBiphenyl CompoundsCell SurvivalHumansMiceBiphenyl CompoundsdiphenyliodoniumN-vinyl-2-pyrrolidinoneOnium CompoundsPyrrolidinonesbioxolographyhigh‐resolutionspatial patterningvolumetric bioprinting

Identifiers

PMID40285580
PMCPMC12447060

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.