Evaluation and Application of the MIRA-qPCR Method for Rapid Detection of Norovirus Genogroup II in Shellfish.

Yanting Zhu, Mengyuan Song, Yingjie Pan, Yong Zhao, Haiquan Liu

Abstract read

In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Yanting ZhuCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

Mengyuan SongCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

Yingjie PanCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

Yong ZhaoCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

Haiquan LiuCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

Funding

Science and Technology Innovation Action Plan" Agricultural Field Project 22N31900600

6 · The paper itself

Abstract

Globally, norovirus has become the primary cause of outbreaks of acute gastroenteritis, and an increasing number of norovirus GII infections have been associated with shellfish. This highlights the urgent need to establish sensitive and rapid detection platforms for timely screening of contaminated shellfish to reduce the risk of virus transmission. To address this challenge, we developed a novel detection method combining multienzyme isothermal rapid amplification (MIRA) with qPCR, referred to as MIRA-qPCR, specifically targeting norovirus GII. It exhibited robust specificity, demonstrating no cross-reactivity with

Indexed as

food safetyMIRA–qPCRnorovirus GIIrapid amplificationshellfish

Identifiers

PMID40284551

DOI10.3390/microorganisms13040712

PMCPMC12029516

What OpenQuestion holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.