Highly Sensitive and Specific Lateral Flow Detection for DNA Methylation Based on GIaI-Mediated Specific-Terminal-Mediated Polymerase Chain Reaction.

Lihui Ke, Hang Zhao, Hongbo Shan, Yicheng Chen, Yongsheng Cai, Yang Wang, Bo Wei, Minghua Du

Abstract read

In one paragraph

Article in Micromachines, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Lihui KeDepartment of Thoracic Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

Hang ZhaoCollege of Health Science and Environmental Engineering, Shenzhen Technology University, Shenzhen 518118, China.

Hongbo ShanAdicon Clinical Laboratories, Inc., Hangzhou 310023, China.

Yicheng ChenKey Laboratory of Biomechanics and Mechanobiology, Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Engineering Medicine, Beihang University, Beijing 100191, China.

Yongsheng CaiDepartment of Thoracic Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

Yang WangKey Laboratory of Biomechanics and Mechanobiology, Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Engineering Medicine, Beihang University, Beijing 100191, China.

Bo WeiDepartment of Thoracic Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

Minghua DuDepartment of Emergency, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.ORCID 0000-0001-6156-3518

Funding

Beijing Natural Science Foundation L246068Beijing Tiantan Hospital Scientific Research Supporting Funds for Introduced Talents YZ-2022-01National Natural Science Foundation of China 62471017, 62371317Shandong Provincial Natural Science Foundation ZR2024QF030

6 · The paper itself

Abstract

Sensitive and specific detection of DNA methylation is crucial for the early diagnosis of various human diseases, particularly cancers. However, conventional methylation detection methods often face challenges in balancing both sensitivity and specificity. In this study, we present a novel approach that integrates the high specificity of methylation-dependent restriction endonuclease (GlaI) digestion with the amplification efficiency of specific terminal-mediated polymerase chain reaction (STEM-PCR). This combination enables selective amplification of methylated DNA, which is then detected through lateral flow detection (LFD), providing a simple, visual readout. As a proof of concept, a STEM-PCR-LFD assay was applied to detect methylated

Indexed as

DNA methylation detectionmethylation-dependent restriction endonucleasesSeptin 9STEM-PCR

Identifiers

PMID40283264

DOI10.3390/mi16040387

PMCPMC12029426

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.