Evidence map›Paper›PMID 40282290›Full record

ArticleBiology2025

Design, Screening, and Impact of sgRNAs Targeting Bovine Prolactin Gene Receptor on Embryonic Development Using Stably Transfected Cell Lines.

Daqing Wang, Guifang Cao, Xin Li, Xin Cheng, Zhihui Guo, Lu Li, Hong Su, Kai Zhang, Yuanyuan Zhang, Min Zhang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Daqing WangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Guifang CaoCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Xin LiCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Xin ChengCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Zhihui GuoCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Lu LiCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Hong SuCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.ORCID 0000-0002-8656-8259
Kai ZhangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.ORCID 0000-0001-5235-4562
Yuanyuan ZhangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.ORCID 0009-0003-3791-8296
Min ZhangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Feifei ZhaoCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Yifan ZhaoCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Junxi LiangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Yiyi LiuCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Yong ZhangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.

Funding

This research was supported by The Biological Breeding Major Project (No. 2023ZD04050） (No. 2023ZD04050）
6 · The paper itself

Abstract

This study designed three sgRNAs (sgRNA139, sgRNA128, and sgRNA109) targeting the prolactin gene receptor (PRLR) in fetal cattle, utilized Cas9 to cleave endogenous DNA, and screened stable cell lines for somatic cell nuclear transfer experiments to investigate the impact of different editing sites on embryonic development. The results showed that sgRNA139 had the highest cleavage efficiency (Fcut = 0.65, Indels = 42.19%), while sgRNA109 had the lowest (Fcut = 0.45, Indels = 35.31%). No significant differences were observed in cell growth status after electroporation (

Indexed as

gene editingPRLRsomatic cell nuclear transfer

Identifiers

PMID40282290
PMCPMC12024835

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.