Evidence map›Paper›PMID 40281994›Full record

ArticleAnimals : an open access journal from MDPI2025

Stability over Time of the Sperm Motility Biomarker proAKAP4 in Repeated Dog Ejaculates.

Giulia Siena, Alain Fontbonne, Barbara Contiero, Cindy Maenhoudt, Guillaume Robiteau, Sarah Slimani, Nicolas Sergeant, Laurent Tiret, Chiara Milani

Abstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Giulia SienaDepartment of Animal Medicine, Production and Health, University of Padova, 35020 Legnaro, PD, Italy.ORCID 0000-0003-3582-2963
Alain FontbonneCentre d'Étude en Reproduction des Carnivores, Ecole Nationale Vétérinaire d'Alfort, 7 Avenue du Général de Gaulle, F-94700 Maisons-Alfort, France.
Barbara ContieroDepartment of Animal Medicine, Production and Health, University of Padova, 35020 Legnaro, PD, Italy.ORCID 0000-0002-6586-5035
Cindy MaenhoudtCentre d'Étude en Reproduction des Carnivores, Ecole Nationale Vétérinaire d'Alfort, 7 Avenue du Général de Gaulle, F-94700 Maisons-Alfort, France.
Guillaume RobiteauCentre d'Étude en Reproduction des Carnivores, Ecole Nationale Vétérinaire d'Alfort, 7 Avenue du Général de Gaulle, F-94700 Maisons-Alfort, France.
Sarah SlimaniCentre d'Étude en Reproduction des Carnivores, Ecole Nationale Vétérinaire d'Alfort, 7 Avenue du Général de Gaulle, F-94700 Maisons-Alfort, France.
Nicolas SergeantSPQI SAS, F-59000 Lille, France.
Laurent TiretUniversité Paris-Est Créteil, Inserm, U955 Institut Mondor de Recherche Biomédicale, Relaix Team, École Nationale Vétérinaire d'Alfort, F-94700 Maisons-Alfort, France.ORCID 0000-0001-8573-8335
Chiara MilaniDepartment of Animal Medicine, Production and Health, University of Padova, 35020 Legnaro, PD, Italy.ORCID 0000-0001-8021-6810

Funding

Agria Insurance CompanyFrench Kennel ClubUniversity of Padova BIRD228871/22
6 · The paper itself

Abstract

ProAKAP4 is a sperm structural protein involved in motility, capacitation, and fertility. Previous studies suggested it as a suitable marker for canine sperm evaluation. Our study aimed to assess proAKAP4 concentration variations among different ejaculates collected from the same dogs. Fourteen male dogs from 12 different breeds, 1-14 years old and 6.9-95 kg bodyweight, underwent semen collection at least twice (1-4 times) during the same or different days. Sperm concentration, morphology, proAKAP4 concentration, total (TM%), and progressive motility (PM%) were considered. ProAKAP4 values were divided into four classes: ≤15, 15-40, 40-60, and >60 ng/10 M spermatozoa. The time interval between two semen collections was classified as: first collection (T0), semen collection performed 2-3 h after the first one (T0.5) and after ≥1 day (1-36 days, T1). Thirty-three ejaculates were collected. A correlation was found between proAKAP4 classes and TM% (r = 0.40,

Indexed as

dogmotility biomarkersproAKAP4semen analysisspermatozoa

Identifiers

PMID40281994
PMCPMC12024009

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.