ArticleBMC plant biology2025
The effect of plant growth regulators, FeO
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- Application of Nanoparticles in Plant In Vitro Culture for Micropropagation and Secondary Metabolite Production: A Review.Plants (Basel, Switzerland) · 2026Review
- Nano-Elicitation Approaches for Boosting Secondary Metabolites in Medicinal Plant Cell Cultures.Plants (Basel, Switzerland) · 2025Review
- Enhancing the accumulation of scopolamine and atropine in Hyoscyamus niger L. callus by LED light and glycine-a cheap method.BMC plant biology · 2025Article
- Nano-elicitation strategy to improve specialized metabolite pathways in plant cell suspension culture.Frontiers in plant science · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundblack seed (Nigella sativa L.) has long been utilized in traditional medicine and as a food ingredient due to its potential therapeutic properties including its effectiveness against cancer, coronaviruses, and bacterial infections. Recently, it has garnered significant attention for its rich reservoir of beneficial secondary metabolites. In vitro culture of black seeds presents an efficient and modern approach for the large-scale production of these valuable compounds, offering advantages such as space efficiency, reduced time, and lower costs. This study aimed to develop and optimize a protocol for callus induction and the identification of key secondary metabolites, including thymoquinone (TQ), phenolic compounds, and flavonoids. To induce callus formation in seed explants, two plant growth regulators (PGRs) were applied individually or in combination and incorporated into Murashige and Skoog (MS) culture medium.
resultsThe combination of Auxin, 2,4-dichlorophenoxyacetic acid (2,4-D) and cytokinin, 6-benzylaminopurine (BAP), effectively induced callus formation in most explants, with the response varying based on concentration. The highest callus fresh weight (7.02 g) was obtained on Red(R) LED lighting with FeO
conclusionThe finding of this study highlights the potential of the proposed method for the large-scale production of secondary metabolites, total carbohydrates, amino acids, phenolic compounds, and flavonoids from black seed callus cultures in a controlled environment. This optimized approach offers a cost-effective and space-efficient strategy for enhancing bioactive compound synthesis, with potential applications in pharmaceutical and nutraceutical industries.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.