Optimization of growth and induction conditions for the production of recombinant whole cell cyclohexanone monooxygenase in Escherichia coli.

Patrik Cabadaj, Viera Illeová, Hana Dobiašová, Marek Bučko, Milan Polakovič

Abstract read

In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Patrik CabadajDepartment of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia.

Viera IlleováDepartment of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia.

Hana DobiašováDepartment of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia.

Marek BučkoDepartment of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 84538, Bratislava, Slovakia.

Milan PolakovičDepartment of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia. milan.polakovic@stuba.sk.

Funding

Agentúra na Podporu Výskumu a Vývoja APVV-20-0272Vedecká Grantová Agentúra MŠVVaŠ SR a SAV VEGA1/0515/22

6 · The paper itself

Abstract

Optimizing biocatalyst production conditions is essential for enhancing productivities and yields in biotransformation applications. This study focused on investigating the impact of the volumetric oxygen mass transfer coefficient (k

Indexed as

Escherichia coliOxygenasesBiocatalysisBioreactorsIsopropyl ThiogalactosideOxygenRecombinant Proteinscyclohexanone oxygenaseIsopropyl ThiogalactosideOxygenOxygenasesRecombinant ProteinsBaeyer-Villiger oxidationCultivation optimizationCyclohexanone monooxygenaseE. coli resting cellsInduction optimizationWhole-cell biocatalyst

Identifiers

PMID40281270

DOI10.1038/s41598-025-99461-3

PMCPMC12032340

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.