Evidence map›Paper›PMID 40280980›Full record

ArticleNPJ biofilms and microbiomes2025

Toxic cultures: e-cigarettes and the oral microbial exposome.

Michelle Lee-Scott Beverly, Prem Prashant Chaudhary, Shareef Majid Dabdoub, Shinae Kim, Emmanouli Chatzakis, Kathryn Williamson, Sukirth Murthy Ganesan, Manoj Yadav, Grace Ratley, Brandon N D'Souza and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NPJ biofilms and microbiomes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
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  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Michelle Lee-Scott BeverlyDepartment of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI, USA.
Prem Prashant ChaudharyEpithelial Therapeutics Unit, National Institute of Allergy and Infectious Diseases Laboratory of Clinical Immunology and Microbiology, IH, Bethesda, MD, USA.
Shareef Majid DabdoubDepartment of Periodontics, School of Dentistry, University of Iowa, Iowa City, IA, USA.ORCID http://orcid.org/0000-0002-1374-8347
Shinae KimBrillient Corporation, Reston, VA, USA.
Emmanouli ChatzakisDepartment of Food Sciences, The Ohio State University, Columbus, OH, USA.
Kathryn WilliamsonDepartment of Food Sciences, The Ohio State University, Columbus, OH, USA.
Sukirth Murthy GanesanDepartment of Periodontics, School of Dentistry, University of Iowa, Iowa City, IA, USA.ORCID http://orcid.org/0000-0002-6303-7287
Manoj YadavEpithelial Therapeutics Unit, National Institute of Allergy and Infectious Diseases Laboratory of Clinical Immunology and Microbiology, IH, Bethesda, MD, USA.
Grace RatleyEpithelial Therapeutics Unit, National Institute of Allergy and Infectious Diseases Laboratory of Clinical Immunology and Microbiology, IH, Bethesda, MD, USA.
Brandon N D'SouzaEpithelial Therapeutics Unit, National Institute of Allergy and Infectious Diseases Laboratory of Clinical Immunology and Microbiology, IH, Bethesda, MD, USA.
Ian A MylesEpithelial Therapeutics Unit, National Institute of Allergy and Infectious Diseases Laboratory of Clinical Immunology and Microbiology, IH, Bethesda, MD, USA.
Purnima S KumarDepartment of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI, USA. kpurnima@umich.edu.ORCID http://orcid.org/0000-0001-5844-1341

Funding

E-cigarettes and perturbations in the subgingival ecosystemR01DE027857 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KUMAR, PURNIMA · 2018 to 2022
$3.2M
NIDCR NIH HHS R01 DE027857U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research (NIDCR) DE027857U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research (NIDCR) DE032895
6 · The paper itself

Abstract

We tested the hypothesis that e-cigarette aerosol is metabolized by the indigenous oral microbiome, leading to structural and functional alterations. We combined untargeted metabolomics of in vitro commensal-rich and pathogen-rich biofilms with metatranscriptomics and fluorescent microscopy and verified the results in human samples. Spectral deconvolution of 4215 peaks identified 969 exposomal and endogenous metabolites that mapped to 23 metabolic pathways. The metabolites clustered by both aerosol characteristics and biofilm composition; and several were verified in human saliva of vapers. E-cigarette exposure upregulated xenobiotic degradation, capsule, peptidoglycan biosynthesis, organic carbon-compound metabolism, antimicrobial resistance, and secretion systems. E-cigarette exposure also altered biofilm architecture characterized by low surface-area to biovolume ratio, high biomass, and diffusion distance. In conclusion, our data suggest that bacterial metabolism of e-cigarette aerosol triggers a quorum-sensing-regulated stress response which mediates the formation of dense, exopolysaccharide-rich biofilms in health-compatible communities and antibiotic resistance and virulence amplification in disease-associated communities.

Indexed as

BacteriaBiofilmsElectronic Nicotine Delivery SystemsMicrobiotaMouthAerosolsHumansMetabolomicsQuorum SensingSalivaVapingAerosols

Identifiers

PMID40280980
PMCPMC12032151

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.