Evidence map›Paper›PMID 40280814›Full record

ArticleTrends in biotechnology2025

A ventilated perfused lung model platform to dissect the response of the lungs to viral infection.

I Deniz Derman, Mecit Altan Alioglu, Joseph Christakiran Moses, Zissis C Chroneos, Yasar Ozer Yilmaz, Dishary Banerjee, Jonathan Koff, Syed Hasan Askari Rizvi, Danielle Nicole Klunk, Nazmiye Celik and 15 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Trends in biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors.

I Deniz DermanEngineering Science and Mechanics Department, Penn State University, University Park, PA 16802, USA; The Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USA.
Mecit Altan AliogluEngineering Science and Mechanics Department, Penn State University, University Park, PA 16802, USA; The Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USA.
Joseph Christakiran MosesEngineering Science and Mechanics Department, Penn State University, University Park, PA 16802, USA; The Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USA.
Zissis C ChroneosDepartment of Pediatrics, Penn State University College of Medicine, Hershey, PA 17033, USA; Department of Cell and Biological Systems, Penn State University College of Medicine, Hershey, PA, USA.
Yasar Ozer YilmazEngineering Science and Mechanics Department, Penn State University, University Park, PA 16802, USA; The Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USA; Department of Nanoscience and Nanoengineering, Istanbul Technical University, Istanbul, Turkey.
Dishary BanerjeeEngineering Science and Mechanics Department, Penn State University, University Park, PA 16802, USA; The Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USA; Department of Cardiology, School of Medicine, University of California San Diego, La Jolla, CA 92093, USA.
Jonathan KoffDepartment of Medicine, Yale University, New Haven, CT 06520, USA.
Syed Hasan Askari RizviEngineering Science and Mechanics Department, Penn State University, University Park, PA 16802, USA; The Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USA.
Danielle Nicole KlunkBiomedical Engineering Department, Penn State University, University Park, PA 16802, USA.
Nazmiye CelikEngineering Science and Mechanics Department, Penn State University, University Park, PA 16802, USA; The Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USA.
Sirisha PochareddyGenome Sciences Core, Penn State University College of Medicine, Hershey, PA 17033, USA.
Todd M UmsteadDepartment of Pediatrics, Penn State University College of Medicine, Hershey, PA 17033, USA.
Ilayda NamliEngineering Science and Mechanics Department, Penn State University, University Park, PA 16802, USA; The Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USA.
Sarah E HoltonTranslational Immunology Department, Benaroya Research Institute, Seattle, WA 98101, USA; Department of Pulmonary, Critical Care, & Sleep Medicine, University of Washington, Seattle, WA 98195, USA.
Carmen MikacenicTranslational Immunology Department, Benaroya Research Institute, Seattle, WA 98101, USA.
Jessica L ThompsonChemistry Department, Penn State University, University Park, PA 16802, USA.
Diana Cadena CastanedaThe Jackson laboratory for Genomic Medicine, Farmington, CT 06032, USA.
Danielle Reifsnyder HickeyChemistry Department, Penn State University, University Park, PA 16802, USA; Department of Materials Science and Engineering, Penn State University, University Park, PA 16802, USA; Materials Research Institute, Penn State University, University Park, PA 16802, USA.
Momoka NagamineThe Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USA; Chemistry Department, Penn State University, University Park, PA 16802, USA.
Prajakta WarangDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Michael SchotsaertDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Phylip ChenCenter for Vaccines and Immunity, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH 43205, USA.
Mark E PeeplesCenter for Vaccines and Immunity, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH 43205, USA; Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA; Infectious Disease Institute, The Ohio State University, Columbus, OH 43210, USA.
Karolina PaluckaThe Jackson laboratory for Genomic Medicine, Farmington, CT 06032, USA.
Ibrahim T OzbolatEngineering Science and Mechanics Department, Penn State University, University Park, PA 16802, USA; The Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USA; Biomedical Engineering Department, Penn State University, University Park, PA 16802, USA; Materials Research Institute, Penn State University, University Park, PA 16802, USA; Cancer Institute, Penn State University, University Park, PA 16802, USA; Neurosurgery Department, Penn State University, University Park, PA 16802, USA. Electronic address: ito1@psu.edu.

Funding

Technology Development Project - Increasing the complexity of ex vivo human airway models for studying immune response to viral infectionU19AI142733 · NIAID · JACKSON LABORATORY · PI Anna Karolina Palucka · 2019 to 2026
$23.1M
High-throughput Spheroid Bioprinting Technology for Scalable Fabrication of TissuesR01EB034566 · NIBIB · PENNSYLVANIA STATE UNIVERSITY, THE · PI Ibrahim Ozbolat · 2023 to 2026
$2.2M
3D Printing of Air: An Intangible Ink for Fabrication of Vascularized TissuesR01EB036245 · NIBIB · PENNSYLVANIA STATE UNIVERSITY, THE · PI Ibrahim Ozbolat, DINO J RAVNIC · 2024 to 2026
$1.7M
NIAID NIH HHS U19 AI142733NIBIB NIH HHS R01 EB034566NIBIB NIH HHS R01 EB036245
6 · The paper itself

Abstract

In this study, we developed a 3D lung model that incorporated alveolar and vascular components, allowing for the investigation of lung physiology and responses to infection. We investigated the role of ventilation in formation of the alveolar epithelial layer and its response to viral infections. We subjected our perfused model to a continuous respiratory cycle at the air-liquid interface (ALI) for up to 10 days. The results revealed that ventilation increased tight-junction formation with better epithelial barrier function over time. Two viruses, influenza and respiratory syncytial virus (RSV), were tested, where ventilation enhanced infectivity with an increased progression of viral spread over time while sensitizing the epithelium for viral recognition. Ventilation also attenuated the production of key proinflammatory chemokines. Our findings represent a critical step forward in advancing our understanding of lung-specific viral responses and respiratory infections in response to ventilation, shedding light on vital aspects of pulmonary physiology and pathobiology.

Indexed as

LungModels, BiologicalRespiratory Syncytial Virus InfectionsAnimalsDogsHumansPerfusionRespiratory Syncytial Viruses3D printingdisease modelinginfectionlung models

Identifiers

PMID40280814
PMCPMC12226239

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.