Evidence map›Paper›PMID 40280138›Full record

ArticleStem cell reports2025

MIXL1 activation in endoderm differentiation of human induced pluripotent stem cells.

Pierre Osteil, Sarah Withey, Nicole Santucci, Nader Aryamanesh, Ignatius Pang, Nazmus Salehin, Jane Sun, Annie Qin, Jiayi Su, Hilary Knowles and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Pierre OsteilEmbryology Research Unit, Children's Medical Research Institute, University of Sydney, Sydney, Australia. Electronic address: pierre.osteil@uca.fr.
Sarah WitheyAustralian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Australia.
Nicole SantucciEmbryology Research Unit, Children's Medical Research Institute, University of Sydney, Sydney, Australia.
Nader AryamaneshBioinformatics Group, Children's Medical Research Institute, University of Sydney, Sydney, Australia.
Ignatius PangBioinformatics Group, Children's Medical Research Institute, University of Sydney, Sydney, Australia.
Nazmus SalehinSchool of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, Australia.
Jane SunEmbryology Research Unit, Children's Medical Research Institute, University of Sydney, Sydney, Australia.
Annie QinEmbryology Research Unit, Children's Medical Research Institute, University of Sydney, Sydney, Australia.
Jiayi SuEmbryology Research Unit, Children's Medical Research Institute, University of Sydney, Sydney, Australia.
Hilary KnowlesEmbryology Research Unit, Children's Medical Research Institute, University of Sydney, Sydney, Australia.
Xiucheng Bella LiEmbryology Research Unit, Children's Medical Research Institute, University of Sydney, Sydney, Australia.
Simon CaiBioinformatics Group, Children's Medical Research Institute, University of Sydney, Sydney, Australia.
Ernst WolvetangAustralian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Australia.
Patrick P L TamEmbryology Research Unit, Children's Medical Research Institute, University of Sydney, Sydney, Australia; School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, Australia. Electronic address: ptam@cmri.org.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human induced pluripotent stem cells (hiPSCs) possess the ability to differentiate into a multitude of cell and tissue types but display heterogeneous propensity of differentiation into specific lineage. Characterization of the transcriptome of 11 hiPSC lines showed that activation of MIXL1 at the early stage of stem cell differentiation correlated with higher efficacy in generating definitive endoderm and advancing differentiation and maturation of endoderm derivatives. Enforced expression of MIXL1 in the endoderm-inefficient hiPSCs enhanced the propensity of endoderm differentiation, suggesting that modulation of key drivers of lineage differentiation can re-wire hiPSC to the desired lineage propensity to generate the requisite stem cell products.

Indexed as

Cell DifferentiationEndodermHomeodomain ProteinsInduced Pluripotent Stem CellsCell LineCell LineageHumansTranscriptomeHomeodomain Proteins2D gastruloidsdifferentiationendodermhuman induced pluripotent stem cellsMIXL1scRNA-seq

Identifiers

PMID40280138
PMCPMC12143138

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.