ArticleJournal of agricultural and food chemistry2025
Boosting Biocatalytic Efficiency: Engineering of Chitinase Chit33 with Chitin and Cellulose Binding Domains for Sustainable Chitin Conversion.
Article in Journal of agricultural and food chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Site-directed mutagenesis and semi-rational design to enhance chitinolytic activity of bacterial exochitinase.Microbial cell factories · 2026Article
- Green bioconversion of insoluble chitin: chitinase development pathways via multi-strategy synergy.Bioresources and bioprocessing · 2026Review
- Highly efficient chitinase production fromFrontiers in microbiology · 2026Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endochitinase Chit33 has shown great potential in converting chitin, a recalcitrant waste, into bioactive chitooligosaccharides (COS). This study evaluates how cellulose-binding domain (CBD) and chitin-binding domain (ChBD) affect the hydrolytic activity and product specificity of Chit33. Recombinant proteins were produced and isolated with a simple yeast extracellular medium concentration. The domain functionality was proved using chitin and cellulose supports. ChBD provided more stable immobilization than CBD but reduced the Chit33 activity. CBD enhanced the enzyme activity on both colloidal (α-/β-allomorphs) and crystalline chitin, doubling it on α-chitin, although not on their deacetylated forms. Besides, CBD increased the COS production from the colloidal forms of α-/β-chitin (by 30% and 85%, respectively) and expanded the product diversity from 1 to 9
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