Evidence map›Paper›PMID 40279390›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

Biofilm architecture determines the dissemination of conjugative plasmids.

Sarah Djermoun, Daniel K H Rode, Eva Jiménez-Siebert, Niklas Netter, Christian Lesterlin, Knut Drescher, Sarah Bigot

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
  6. The Antimicrobial Peptide CRAMP-34 EradicatesAntibiotics (Basel, Switzerland) · 2026
    Article
  7. Article
  8. Article
  9. Article
  10. mSystems · 2025
    Article
  11. Fluid flow generates bacterial conjugation hot spots by increasing the rate of shear-driven cell-cell encounters.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  12. The extended mobility of plasmids.Nucleic acids research · 2025
    Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Sarah Djermoun *Microbiologie Moléculaire et Biochimie Structurale, Université Lyon 1, CNRS, Inserm, Lyon 69007, France.
Daniel K H Rode *Biozentrum, University of Basel, Basel 4056, Switzerland.
Eva Jiménez-SiebertBiozentrum, University of Basel, Basel 4056, Switzerland.
Niklas NetterBiozentrum, University of Basel, Basel 4056, Switzerland.
Christian LesterlinMicrobiologie Moléculaire et Biochimie Structurale, Université Lyon 1, CNRS, Inserm, Lyon 69007, France.
Knut DrescherBiozentrum, University of Basel, Basel 4056, Switzerland.ORCID 0000-0002-7340-2444
Sarah BigotMicrobiologie Moléculaire et Biochimie Structurale, Université Lyon 1, CNRS, Inserm, Lyon 69007, France.ORCID 0000-0002-9340-6303

Funding

Agence Nationale de la Recherche (ANR) ANR-19-ARMB-0006-01Association Vaincre la Mucoviscidose (French CF Association) RF20200502684Bundesministerium für Bildung und Forschung (BMBF) 16GW0245Deutsche Forschungsgemeinschaft (DFG) DR 982/6-1 part of SPP 2389Deutscher Akademischer Austauschdienst France (DAAD France) 57552336EC | H2020 | PRIORITY 'Excellent science' | H2020 Marie Skłodowska-Curie Actions (MSCA) 955910Germaine de Stael swiss Academy of Engineering Sciences 47902YJSwiss National Science Foundation TMCG-3 _ 213801
6 · The paper itself

Abstract

Plasmid conjugation is a contact-dependent horizontal gene transfer mechanism that significantly contributes to the dissemination of antibiotic resistance among bacteria. While the molecular mechanisms of conjugation have been extensively studied, our understanding of plasmid transfer dynamics within spatially structured bacterial communities and the influence of community architecture on plasmid dissemination remains limited. In this study, we use live-cell fluorescence microscopy to investigate the propagation of the broad host range RP4 conjugative plasmid in

Indexed as

BiofilmsConjugation, GeneticEscherichia coliPlasmidsGene Transfer, HorizontalMicroscopy, Fluorescenceantibiotic resistancebacterial conjugationbiofilmhorizontal gene transferlive-cell fluorescence microscopy

Identifiers

PMID40279390
PMCPMC12054802

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.