ArticleThe Journal of infectious diseases2025
Cellular Tropism and Viral Genetics in Appendix Tissue Reservoirs of Subtype C HIV-1-Infected Aviremic Persons.
Article in The Journal of infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
backgroundAlthough antiretroviral therapy (ART) can suppress plasma viral loads in people living with human immunodeficiency virus (HIV), it cannot eliminate HIV-1 proviruses persisting in various tissue reservoirs, and HIV-1 will rebound after ART cessation. We have identified the appendix as a novel HIV tissue reservoir, but nothing is known about the provirus and the cellular tropism in this tissue.
methodsWe used single-genome analysis of HIV-1 env DNA and compared the viral sequences from appendix tissues and lymph nodes of the same people living with HIV-1. Sequential multiplex immunofluorescence labeling and RNAscope in situ hybridization on formalin-fixed, paraffin-embedded appendix sections were performed to identify cell types harboring HIV-1 proviral DNA in the appendix, by visualizing viral and cellular biomarkers colocalization.
resultsThe viral populations from the appendix were less heterogeneous than those from the lymph nodes in aviremic individuals, suggesting there is reduced ART selective pressure or the presence of appendix tropic HIV-1. Furthermore, we demonstrated that follicular dendritic cell (FDCs), rather than CD4+ T cells within the appendix, are the major cell type harboring subtype C HIV-1. Notably, although some subtype C HIV-1 signals could be remaining virions captured by FDCs even in aviremic individuals, HIV-1 proviral DNA was detected in some FDCs in the appendix tissues of aviremic individuals, indicating the presence of infected FDCs.
conclusionsThese results highlight the importance of analyzing all potential tissue and cellular tropism across different HIV-1 subtypes, to inform tailored therapeutic strategies for the diverse reservoirs that may differ across subtypes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.