Evidence map›Paper›PMID 40278663›Full record

ArticleToxins2025

Aflatoxin M1 Determination in Whole Milk with Immersible Silicon Photonic Immunosensor.

Dimitra Kourti, Michailia Angelopoulou, Eleni Makarona, Anastasios Economou, Panagiota Petrou, Konstantinos Misiakos, Sotirios Kakabakos

Abstract read
In one paragraph

Article in Toxins, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Dimitra KourtiImmunoassays-Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR "Demokritos", GR-15341 Agia Paraskevi, Greece.
Michailia AngelopoulouImmunoassays-Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR "Demokritos", GR-15341 Agia Paraskevi, Greece.
Eleni MakaronaInstitute of Nanoscience & Nanotechnology, NCSR "Demokritos", GR-15341 Agia Paraskevi, Greece.ORCID 0000-0002-5115-5518
Anastasios EconomouAnalytical Chemistry Lab, Department of Chemistry, National and Kapodistrian University of Athens, GR-15771 Panepistimiopolis Zografou, Greece.ORCID 0000-0003-4590-9261
Panagiota PetrouImmunoassays-Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR "Demokritos", GR-15341 Agia Paraskevi, Greece.ORCID 0000-0001-9125-3625
Konstantinos MisiakosInstitute of Nanoscience & Nanotechnology, NCSR "Demokritos", GR-15341 Agia Paraskevi, Greece.
Sotirios KakabakosImmunoassays-Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR "Demokritos", GR-15341 Agia Paraskevi, Greece.ORCID 0000-0002-0043-3119

Funding

European Regional Development Fund Τ2ΕΔΚ-01934/FOODSENSEuropean Union Horizon 2020 Research and Innovation program 101007299 (SAFEMILK)
6 · The paper itself

Abstract

Aflatoxin M1 (AFM1) appears in the milk of animals that have consumed feed contaminated with aflatoxin B1. AFM1 presence in milk is regulated by the European Commission, which has set the maximum allowable limits for adult and infant consumption to 50 and 25 pg/mL, respectively. Here, a rapid and sensitive method for detecting AFM1 in milk based on an immersible silicon photonic chip is presented. The chip features two U-shaped silicon nitride waveguides formed as Mach-Zehnder interferometers. One interferometer is functionalized with AFM1-bovine serum albumin conjugate and the other with BSA to serve as a blank. The chip is connected to a broad-band white LED and a spectrophotometer by a bifurcated optical fiber and an assay is performed by immersing the chip in a mixture of milk with the anti-AFM1 antibody. Then, the chip is sequentially immersed in biotinylated anti-rabbit IgG antibody and streptavidin solutions for signal enhancement. The assay is completed in 20 min and the detection limit for AFM1 in undiluted milk is 20 pg/mL. Given its analytical performance and the absence of pumps and fluidics that lead to a compact instrument design, the proposed immunosensor is ideal for the on-site detection of AFM1 in milk samples.

Indexed as

Aflatoxin M1Biosensing TechniquesFood ContaminationMilkAnimalsCattleImmunoassaySiliconAflatoxin M1Siliconaflatoxin M1immunosensorMach–Zehnder interferometrymilk safetywhole milk

Identifiers

PMID40278663
PMCPMC12031367

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.