Evidence map›Paper›PMID 40278511›Full record

ArticleMethods and protocols2025

Enzyme-Linked Immunosorbent Assay (ELISA) Development for Equine Serum Amyloid A (SAA) Determination Using Recombinant Proteins.

Pollyanna C Souto, Marcus R Santos, Andrés M Ortega Orozco, Lucas D Bento, Camilo J Ramirez-Lopez, Fabrícia M Girardi, Júlia C Assis Machado, Leandro L de Oliveira, Leandro A da Fonseca

Abstract read
In one paragraph

Article in Methods and protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Pollyanna C SoutoLaboratory of Veterinary Clinical Pathology, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.ORCID 0000-0003-2357-5344
Marcus R SantosLaboratory of Immunobiologics and Bacteriosis, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.
Andrés M Ortega OrozcoLaboratory of Veterinary Clinical Pathology, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.ORCID 0000-0002-8453-0484
Lucas D BentoLaboratory of Veterinary Clinical Pathology, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.
Camilo J Ramirez-LopezLaboratory of Animal Reproduction, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.ORCID 0000-0002-6944-7287
Fabrícia M GirardiLaboratory of Veterinary Clinical Pathology, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.
Júlia C Assis MachadoLaboratory of Veterinary Clinical Pathology, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.ORCID 0000-0002-2870-7468
Leandro L de OliveiraLaboratory of Immunology and Glycobiology, Department of Biology, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.ORCID 0000-0003-4353-7011
Leandro A da FonsecaLaboratory of Veterinary Clinical Pathology, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.ORCID 0000-0001-5296-6657

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We aimed to develop a species-specific ELISA for qualitatively and quantitatively determining serum amyloid A (SAA) in horses. Current methods for measuring SAA in horses utilize ELISA or immunoturbidimetric tests designed for human SAA, which are not specific to horses. Mice and rabbits were used to generate polyclonal antibodies against equine SAA. The study examined serum samples from 32 horses with acute inflammatory disease (SG) and 25 clinically healthy horses. Furthermore, the SAAeq kinetics were observed in three horses from the SG group at three different timepoints. The SAA-ELISA established a cut-off at 0.06 OD

Indexed as

acute-phasehorseimmunoassayinflammationprotein

Identifiers

PMID40278511
PMCPMC12029847

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.