Evidence map›Paper›PMID 40278226›Full record

ArticleJournal of functional biomaterials2025

Fabrication of a 3D Corneal Model Using Collagen Bioink and Human Corneal Stromal Cells.

Alexander J Choi, Brenna S Hefley, Hannah A Strobel, Sarah M Moss, James B Hoying, Sarah E Nicholas, Shadi Moshayedi, Jayoung Kim, Dimitrios Karamichos

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of functional biomaterials, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Keratin-Laden Bioink for Corneal Stroma Bioprinting.Bioengineering (Basel, Switzerland) · 2026
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Alexander J ChoiNorth Texas Eye Research Institute, University of North Texas Health Science Center, 3430 Camp Bowie Blvd, Fort Worth, TX 76107, USA.ORCID 0009-0003-3071-6747
Brenna S HefleyNorth Texas Eye Research Institute, University of North Texas Health Science Center, 3430 Camp Bowie Blvd, Fort Worth, TX 76107, USA.ORCID 0000-0002-8953-5102
Hannah A StrobelAdvanced Solutions Life Sciences, 500 N Commercial St., Manchester, NH 03101, USA.
Sarah M MossAdvanced Solutions Life Sciences, 500 N Commercial St., Manchester, NH 03101, USA.
James B HoyingAdvanced Solutions Life Sciences, 500 N Commercial St., Manchester, NH 03101, USA.ORCID 0000-0001-7959-227X
Sarah E NicholasNorth Texas Eye Research Institute, University of North Texas Health Science Center, 3430 Camp Bowie Blvd, Fort Worth, TX 76107, USA.
Shadi MoshayediNorth Texas Eye Research Institute, University of North Texas Health Science Center, 3430 Camp Bowie Blvd, Fort Worth, TX 76107, USA.
Jayoung KimNorth Texas Eye Research Institute, University of North Texas Health Science Center, 3430 Camp Bowie Blvd, Fort Worth, TX 76107, USA.ORCID 0000-0002-4883-2768
Dimitrios KaramichosNorth Texas Eye Research Institute, University of North Texas Health Science Center, 3430 Camp Bowie Blvd, Fort Worth, TX 76107, USA.ORCID 0000-0002-8761-3824

Funding

University of North Texas Health Science Center UNTHSC Team Science Grant
6 · The paper itself

Abstract

Corneal transplantation remains a critical treatment option for individuals with corneal disorders, but it faces challenges such as rejection, high associated medical costs, and donor scarcity. A promising alternative for corneal replacement involves fabricating artificial cornea from a patient's own cells. Our study aimed to leverage bioprinting to develop a corneal model using human corneal stromal cells embedded in a collagen-based bioink. We generated both cellular and acellular collagen I (COL I) constructs. Cellular constructs were cultured for up to 4 weeks, and gene expression analysis was performed to assess extracellular matrix (ECM) remodeling and fibrotic markers. Our results demonstrated a significant decrease in the expression of COL I, collagen III (COL III), vimentin (VIM), and vinculin (VCL), indicating a dynamic remodeling process towards a more physiologically relevant corneal ECM. Overall, our study provides a foundational framework for developing customizable, corneal replacements using bioprinting technology. Further research is necessary to optimize the bioink composition and evaluate the functional and biomechanical properties of these bioengineered corneas.

Indexed as

bioengineeringbioinkcorneacorneal bioprintingcorneal fibrosisECM

Identifiers

PMID40278226
PMCPMC12028034

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.