Evidence map›Paper›PMID 40277884›Full record

ArticleCells2025

Pro-Inflammatory Properties of Salivary Gland-Derived Fibroblasts-Implications in Sjögren's Disease.

Matthias Brunner, Daniel Guggisberg, Marco Sprecher, Ondrej Pastva, Kristina Bürki, Miranda Houtman, Marco Kreuzer, Sara Andrea Krättli, Laura Jahnke, Mila Roceri and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Matthias BrunnerDepartment of Rheumatology and Immunology, Inselspital, Bern University Hospital, University of Bern, 3008 Bern, Switzerland.ORCID 0000-0002-6781-6210
Daniel GuggisbergDepartment of Rheumatology and Immunology, Inselspital, Bern University Hospital, University of Bern, 3008 Bern, Switzerland.
Marco SprecherDepartment of Rheumatology, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland.
Ondrej PastvaLung Precision Medicine (LPM), Department for BioMedical Research, 3008 Bern, Switzerland.ORCID 0000-0001-7456-3584
Kristina BürkiDepartment of Rheumatology, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland.
Miranda HoutmanDepartment of Rheumatology, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland.ORCID 0000-0001-5317-0327
Marco KreuzerInterfaculty Bioinformatics Unit, University of Bern, 3012 Bern, Switzerland.ORCID 0000-0001-6784-0854
Sara Andrea KrättliInterfaculty Bioinformatics Unit, University of Bern, 3012 Bern, Switzerland.ORCID 0009-0000-4679-3112
Laura JahnkeLung Precision Medicine (LPM), Department for BioMedical Research, 3008 Bern, Switzerland.ORCID 0000-0001-7762-5162
Mila RoceriDepartment of Rheumatology and Immunology, Inselspital, Bern University Hospital, University of Bern, 3008 Bern, Switzerland.
Rémy BruggmannInterfaculty Bioinformatics Unit, University of Bern, 3012 Bern, Switzerland.ORCID 0000-0001-5629-6363
Muriel ElhaiDepartment of Rheumatology, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland.
Britta MaurerDepartment of Rheumatology and Immunology, Inselspital, Bern University Hospital, University of Bern, 3008 Bern, Switzerland.
Thomas M MartiLung Precision Medicine (LPM), Department for BioMedical Research, 3008 Bern, Switzerland.ORCID 0000-0003-3005-220X
Caroline OspeltDepartment of Rheumatology, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland.
Kerstin KleinDepartment of Rheumatology and Immunology, Inselspital, Bern University Hospital, University of Bern, 3008 Bern, Switzerland.ORCID 0000-0001-5303-8027

Funding

Medical Faculty of the University of Bern SF Board CallSwiss Life JubiläumsstiftungUniversity of Bern UniBe ID grant
6 · The paper itself

Abstract

Salivary gland dysfunction is a hallmark of Sjögren's disease (SjD). Here, we investigated the pro-inflammatory properties of salivary gland-derived fibroblasts (SGF) that were cultured from minor salivary gland (MSG) tissues of patients with SjD and controls. SGF from patients with SjD exhibited higher rates of proliferation compared to controls. RNA sequencing revealed pronounced pro-inflammatory properties of SGF in response to stimulation with IL1 and polyI:C, with an activation of "interferon responses", "JAK STAT", and "NF-kappa B" signaling, as well as "complement" pathways. In addition to encoding pro-inflammatory transcripts, stimulated SGF featured increased expression of a number of non-coding enhancer RNAs (eRNAs) that we originally identified in TNF-stimulated synovial fibroblasts (FLS) by CAGE sequencing. We confirmed the expression of selected eRNAs in SGF and FLS through time-course experiments upon stimulation with different pro-inflammatory stimuli using real-time PCR. Furthermore, we detected eRNAs for IL6 (eIL6) and IL8 (eIL8#3) in MSG tissues. Treatment of SGF with the bromodomain inhibitor I-BET suppressed IL1- and LPS-induced expression of all eRNAs tested, as well as their associated pro-inflammatory coding transcripts. Transfection of SGF with antisense nucleotides targeting eCCL20 reduced the LPS-induced expression of this eRNA, as well as CCL20 expression and secretion. Together, our data highlight similarities between SGF and FLS regarding their activation under inflammatory conditions.

Indexed as

FibroblastsInflammationSalivary GlandsSjogren's SyndromeCell ProliferationCells, CulturedFemaleHumansMiddle AgedSignal Transductionbromodomainenhancerfibroblastinflammationsalivary glandSjögren’s disease

Identifiers

PMID40277884
PMCPMC12025495

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.