FRET Visualization of High Mechanosensation of von Willebrand Factor to Hydrodynamic Force.

Mingxing Ouyang, Yao Gao, Binqian Zhou, Jia Guo, Lei Lei, Yingxiao Wang, Linhong Deng

Abstract read

In one paragraph

Article in Biosensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

From biomimicry to clinical actionability: rethinking high-shear thrombosis as a mechanobiological system.Current opinion in hematology · 2026
Review

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mingxing OuyangInstitute of Biomedical Engineering and Health Sciences, School of Medical and Health Engineering, Changzhou University, 1 Gehu Rd, Wujin District, Changzhou 213164, China.ORCID 0000-0002-2910-0713

Yao GaoInstitute of Biomedical Engineering and Health Sciences, School of Medical and Health Engineering, Changzhou University, 1 Gehu Rd, Wujin District, Changzhou 213164, China.

Binqian ZhouInstitute of Biomedical Engineering and Health Sciences, School of Medical and Health Engineering, Changzhou University, 1 Gehu Rd, Wujin District, Changzhou 213164, China.

Jia GuoInstitute of Biomedical Engineering and Health Sciences, School of Medical and Health Engineering, Changzhou University, 1 Gehu Rd, Wujin District, Changzhou 213164, China.

Lei LeiShu Chien-Gene Lay Department of Bioengineering, and Institute of Engineering in Medicine, University of California at San Diego, La Jolla, CA 92093, USA.

Yingxiao WangShu Chien-Gene Lay Department of Bioengineering, and Institute of Engineering in Medicine, University of California at San Diego, La Jolla, CA 92093, USA.

Linhong DengInstitute of Biomedical Engineering and Health Sciences, School of Medical and Health Engineering, Changzhou University, 1 Gehu Rd, Wujin District, Changzhou 213164, China.ORCID 0000-0003-1970-8239

Funding

National Natural Science Foundation of China 12372312, 12272063

6 · The paper itself

Abstract

von Willebrand factor (vWF) is a large glycoprotein in the circulation system, which senses hydrodynamic force at vascular injuries and then recruits platelets in assembling clots. How vWF mechanosenses shear flow for molecular unfolding is an important topic. Here, a Förster resonance energy transfer (FRET) biosensor was developed to monitor vWF conformation change to hydrodynamic force. The vWF-based biosensor is anchored on the cell surface, in which the A2 domain is flanked with a FRET pair. With 293T cells seeded into microfluidic channels, 2.8 dyn/cm

Indexed as

Biosensing TechniquesFluorescence Resonance Energy Transfervon Willebrand FactorHEK293 CellsHumansHydrodynamicsvon Willebrand Factorfluid shearFörster resonance energy transfer (FRET)hemophiliamechanosensationmolecular unfoldingvon Willebrand factor

Identifiers

PMID40277561

DOI10.3390/bios15040248

PMCPMC12026062

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.