ArticleBiosensors2025
Sea Urchin-like Magnetic Microbeads-Based Electrochemical Biosensor for Highly Sensitive Detection of Metabolites.
Article in Biosensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Analyzing metabolite levels in bodily fluids is essential for disease diagnosis and surveillance. Electrochemical biosensors are ideal for monitoring metabolite levels due to their high sensitivity, rapid response, and low cost. The magnetic microbeads-based electrode functionalization method further promotes the automation development of electrochemical biosensors by eliminating the tedious electrode polishing process. In this study, we presented sea urchin-like magnetic microbeads (SMMBs) and constructed an SMMB-based electrochemical biosensor. The specific morphology of SMMBs provides a larger specific surface area and abundant enzyme binding sites, thereby expanding the active reaction interface on the electrode and improving the sensitivity of the biosensor. Experiment results demonstrated that the SMMB-based electrochemical biosensor achieves μM level detection sensitivity for glucose. Furthermore, by replacing the anchored oxidase on SMMBs, the biosensor can be extended to detect other metabolites, such as cholesterol. In summary, the SMMBs provide a new path to handily construct electrochemical biosensors and hold a great potential for metabolite detection and further development.
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