ArticleFrontiers in veterinary science2025
Proteomic analysis of Toraya buffalo seminal plasma and sperm: uncovering insights to optimize reproductive success.
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Current Applications and Future Prospects of High-ThroughputAnimals : an open access journal from MDPI · 2026Review
- Cervical mucus proteomics approach for swamp buffalo secreted fertility biomarker identification.Journal of veterinary research · 2026Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The characterization of sperm and seminal plasma proteins is essential for understanding bull fertility and optimizing reproductive success in buffalo bulls. Despite its importance, the reproductive proteomic of Toraya buffalo, an indigenous breed in Indonesia, remains largely unexplored. This study aimed to examine the seminal plasma and sperm proteins of Toraya buffalo to uncover those critical for reproductive functions. Semen samples were collected from eight Toraya buffalo bulls aged 4 to 10 years. Protein profiling was performed using one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D-SDS-PAGE), followed by in-gel digestion and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Bioinformatics tools, including UniProt, PANTHER, DAVID, and STRING, were utilized to identify and annotate the detected proteins. This study successfully identified four key reproductive proteins: ADAM32 in seminal plasma and ZPBP, SPACA3, and CCDC136 in sperm. These proteins are essential for sperm motility, energy production, and acrosome formation, which are critical processes for fertilization. Additionally, many identified proteins were associated with metabolic pathways, particularly the tricarboxylic acid (TCA) cycle, which plays a fundamental role in energy supply for sperm function. In conclusion, this study offers the first comprehensive proteomic identification of seminal plasma and sperm proteins associated with reproductive functions in the Toraya buffalo. The findings highlight the presence of key proteins in sperm, including ZPBP, SPACA3, and CCDC136, as well as the identification of ADAM32 in seminal plasma, contributing to a deeper understanding of buffalo reproductive biology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.