Evidence map›Paper›PMID 40276050›Full record

ArticleTropical life sciences research2025

Preliminarily Study on Hydroxyproline Content of Purple-spotted Bigeye (

Widiyanto, Uju, Sitti Hardiyanti Rachman, Mala Nurilmala

Abstract read
In one paragraph

Article in Tropical life sciences research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

WidiyantoDepartment of Aquatic Product Technology, Faculty of Fisheries and Marine Science, Bogor Agricultural University. Dramaga, Bogor, West Java 16680, Indonesia.
UjuDepartment of Aquatic Product Technology, Faculty of Fisheries and Marine Science, Bogor Agricultural University. Dramaga, Bogor, West Java 16680, Indonesia.
Sitti Hardiyanti RachmanStudy Program of Fisheries Product Technology, Faculty of Fisheries and Marine Science, Lambung Mangkurat University, A. Yani, Banjarbaru, South Kalimantan 70714, Indonesia.
Mala NurilmalaDepartment of Aquatic Product Technology, Faculty of Fisheries and Marine Science, Bogor Agricultural University. Dramaga, Bogor, West Java 16680, Indonesia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The investigation of alternative raw materials for gelatine production from fishery industry by-products has gained attention due to the increasing demand for gelatine and the importance for sustainable practices. This study aims to determine the optimal hydrochloric acid (HCl) concentration for mineral removal during pre-treatment, assess hydroxyproline content at various processing stages and characterise the resultant gelatine. The methodology involved pre-treatment of the materials with 0.1 M sodium hydroxide (NaOH) to remove non-collagen proteins, followed by mineral extraction using varying HCl concentrations (0.25, 0.5, 0.75 and 1 M). The process included swelling in 0.2% citric acid for 12 h and gelatine extraction at 65°C for 7 h. The results indicated that 0.25 M HCl was most effective for mineral removal. The hydroxyproline analysis showed an insignificant increase (0.088 mg/mL-0.103 mg/mL) from the pre-treatment stage to the final gelatine product. The physicochemical properties of the liquid gelatine, including yield (6.5 ± 0.39%), pH (6.55 ± 0.11), and gel bloom strength (174 ± 8.54 blooms) conformed to Gelatin Manufacturers Institute of America (GMIA). Functional groups confirmed the presence of gelatine-specific, such as amides A, B, I, II and III. The molecular profile is comparable to commercial gelatine, with α1 chains at 130 kDa, α2 chains at 115 kDa, and β chains at 235 kDa. The gelatine derived from the scaly skin of purple-spotted bigeye exhibits promising attributes, aligning with commercial standards and highlights the potential of fishery by-products as a sustainable and halal source of gelatine.

Indexed as

Fish Scaly SkinGelatineHalalHydroxyprolinePurple-spotted

Identifiers

PMID40276050
PMCPMC12017279

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.