ArticleMarine biotechnology (New York, N.Y.)2025
Establishment and Characterization of Hepatocyte Line from Turbot (Scophthalmus maximus L.) and Its Application in the Study of Glucose Metabolism.
Article in Marine biotechnology (New York, N.Y.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
The present study was to establish a hepatocyte line and investigate its role in glucose metabolism. A continuous cell line, THL (turbot hepatocyte line), was established from the liver tissue of turbot (Scophthalmus maximus L.). It has been successfully passaged more than 60 generations. The THL cells showed an epithelial-like morphology and the normal chromosome number was 44. Different methods were used to identify the hepatocytes. Periodic acid-Schiff (PAS) staining for THL cells was positive, and two key functional proteins of hepatocytes, cytokeratin- 18 (CK- 18) and albumin (ALB), were detected in THL cells. The results of CCK- 8 indicated that a medium containing 15 mM glucose showed optimal cell viability of THL. Conversely, elevating glucose concentrations beyond 50 mM markedly impaired THL cell viability. Western blot and qRT-PCR were employed to assess the gene and protein expression in cells treated with varying concentrations of glucose. The results of cells incubated with 0 mM, 15 mM, and 50 mM glucose concentrations showed that compared with the 0 mM glucose group, 15 mM glucose could increase the gene expression of glucokinase (gk) and decrease the gene expression of cytosolic phosphoenolpyruvate carboxykinase (cpepck), mitochondrial phosphoenolpyruvate (mpepck), glucose- 6-phosphatase 1 (g6pase1), forkhead box o1 (foxo1), and glucose-regulated protein 78 (grp78). Compared to 15 mM glucose treatment, the expression of gk in the 50 mM group was significantly decreased, but the expression of cpepck, mpepck, g6pase1, foxo1, and grp78 was significantly increased. Moreover, the protein expression of FoxO1 and GRP78 in 50 mM treatment group was significantly increased compared to that in the15 mM group. In the present study, it was found that excessive glucose level can activate the pathways of FoxO1-mediated gluconeogenesis and GRP78-mediated endoplasmic reticulum stress and reduce the glycolytic pathway, thus disrupting the glucose homeostasis in hepatocytes.
Indexed as
Identifiers
40272575What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.