ArticleMicrobiology spectrum2025
Establishment of a two-dimensional PCR method for simultaneous detection of nine sexually transmitted disease pathogens: insights into coinfection rates and epidemiological trends in HPV screening.
Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Detection of Genital Pathogens and Co-Infections by Multiplex RT-qPCR: Associations with HIV Positivity and Demographic Factors.Diagnostics (Basel, Switzerland) · 2026Article
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Authors and funding
5 authors.
Funding
Abstract
This study developed a two-dimensional PCR (2D-PCR) method for simultaneous detection of nine sexually transmitted disease pathogens (STDPs) in cervical brush samples collected after human papillomavirus (HPV) screening, aiming to evaluate co-infection rates and epidemiological trends. The 2D-PCR assay was optimized under single-tube closed conditions, with sensitivity and accuracy validated. The prevalent nine STDPs were assessed in 2,193 females undergoing routine gynecological inspections and HPV screening. Statistical analysis revealed correlations between HPV genotypes and identified pathogens. Results showed detection limits (LODs) of 10²-10³ copies/μL for STDPs, with high concordance to triplex real-time PCR (Kappa = 0.90). Overall, 48.6% of samples tested positive for ≥1 pathogen, with 36.02% positive for ≥1 STDP. The most prevalent STDPs were IMPORTANCE: This study introduces the first single-tube 2D-PCR method for efficient, high-throughput detection of nine STDPs, addressing a critical gap in co-infection diagnostics. The high prevalence of STDPs and their strong association with human papillomavirus (HPV) infection underscore the clinical relevance of co-pathogen screening, particularly in HPV-positive populations. The significant linkage between
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