Evidence map›Paper›PMID 40270202›Full record

ArticleHaematologica2025

Production of platelets

Holly R Foster, Maria Colzani, Guenaelle Bouet, Daniel Howard, Christian A Di Buduo, Nicole Müller-Sienerth, Amie K Waller, Yi Sun, Natalia Davidenko, Jennifer H Shepherd and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Haematologica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Inside-to-outside and back to the future of megakaryopoiesis.Research and practice in thrombosis and haemostasis · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Holly R FosterWellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge, CB2 0AW.
Maria ColzaniWellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge, CB2 0AW.
Guenaelle BouetWellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge, CB2 0AW.
Daniel HowardWellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge, CB2 0AW.
Christian A Di BuduoDepartment of Molecular Medicine, University of Pavia, Pavia.
Nicole Müller-SienerthCell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Cambridge, CB10 1SA.
Amie K WallerWellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge, CB2 0AW.
Yi SunDepartment of Materials Science and Metallurgy, Cambridge Centre for Medical Materials, University of Cambridge, Cambridge.
Natalia DavidenkoDepartment of Materials Science and Metallurgy, Cambridge Centre for Medical Materials, University of Cambridge, Cambridge.
Jennifer H ShepherdDepartment of Materials Science and Metallurgy, Cambridge Centre for Medical Materials, University of Cambridge, Cambridge.
Thomas MoreauWellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge, CB2 0AW.
Amanda L EvansWellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge, CB2 0AW.
Paolo M SopranoDepartment of Molecular Medicine, University of Pavia, Pavia.
Martin E M ParsonsSPHERE research group, UCD Conway Institute, University College Dublin, Dublin, Ireland; School of Biomolecular and Biomedical Science, University College Dublin, Dublin.
Yumi Ying SimsWellcome Trust Sanger Institute, Cambridge, CB10 1SA.
Meera ArumugamWellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge, CB2 0AW.
Wardiya Afshar-SaberWellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge, CB2 0AW.
Ernest TurroDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029.
Patricia B MaguireSPHERE research group, UCD Conway Institute, University College Dublin, Dublin, Ireland; School of Biomolecular and Biomedical Science, University College Dublin, Dublin.
Serena M BestDepartment of Materials Science and Metallurgy, Cambridge Centre for Medical Materials, University of Cambridge, Cambridge.
Ruth E CameronDepartment of Materials Science and Metallurgy, Cambridge Centre for Medical Materials, University of Cambridge, Cambridge.
Alessandra BalduiniDepartment of Molecular Medicine, University of Pavia, Pavia, Italy; Department of Biomedical Engineering, Tufts University, Medford, MA. alessandra.balduini@unipv.it.
Gavin J WrightCell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Cambridge, CB10 1SA, UK; Department of Biology, Hull York Medical School, York Biomedical Research Institute, University of York, Wentworth Way, York, YO10 5DD.
Cedric GhevaertWellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge, CB2 0AW. cg348@cam.ac.uk.

Funding

In vitro bioreactor system for platelet formationR01EB016041 · NIBIB · TUFTS UNIVERSITY MEDFORD · PI BALDUINI, ALESSANDRA, KAPLAN, DAVID L. · 2012 to 2020
$2.7M
Medical Research Council MR/ L022982/1NIBIB NIH HHS R01 EB016041Wellcome TrustWellcome Trust 206194
6 · The paper itself

Abstract

The safety, quality and supply of donor-derived platelet units intended for transfusion have improved over the past decades but significant problems still remain. In vitro-derived platelets offer a possible alternative but up-scaling production is hindered by our limited understanding of thrombopoiesis (the release of platelets by their mother cell, the megakaryocyte [MK]). Here, we have developed an integrated strategy aiming to mimic ex vivo the bone marrow physiological niche that promotes thrombopoiesis by mature MK. The screening of a panel of 259 recombinant transmembrane proteins derived from cells known to promote platelet production through direct contact with MK enabled us to show that ACVR1B, CRTAM, MUCEN and BTN1A1 improve platelet production from either cord blood- (ACVR1B) or pluripotent stem cells-derived (CRTAM, MUCEN and BTN1A1) MK. Using two different methodologies, we functionalize either collagen- or silk-based 3-dimensional scaffolds and confirm increased functional platelet production by up to 2-fold. This unbiased approach has allowed us to identify novel proteins whose role in platelet formation was previously unknown and highlights the potential gain of recreating the MK niche to allow in vitro platelets to become a viable alternative for transfusion.

Indexed as

Blood PlateletsBone MarrowStem Cell NicheThrombopoiesisTissue ScaffoldsCells, CulturedFetal BloodHumansMegakaryocytes

Identifiers

PMID40270202
PMCPMC12485342

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.