Evidence map›Paper›PMID 40270183›Full record

ArticleJournal of cell science2025

Enhancement of CEP215 dynamics for spindle pole assembly during mitosis.

Hyunjeong Yoo, Taehyun Kim, Sungjin Ryu, Donghee Ko, Jeesoo Kim, Hee-Jung Choi, Yongdae Shin, Kunsoo Rhee

Abstract read
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In one paragraph

Article in Journal of cell science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hyunjeong YooDepartment of Biological Sciences, Seoul National University, Seoul 08826, Korea.
Taehyun KimDepartment of Mechanical Engineering, Seoul National University, Seoul 08826, Korea.
Sungjin RyuDepartment of Biological Sciences, Seoul National University, Seoul 08826, Korea.
Donghee KoDepartment of Biological Sciences, Seoul National University, Seoul 08826, Korea.
Jeesoo KimDepartment of Biological Sciences, Seoul National University, Seoul 08826, Korea.
Hee-Jung ChoiDepartment of Biological Sciences, Seoul National University, Seoul 08826, Korea.
Yongdae ShinDepartment of Mechanical Engineering, Seoul National University, Seoul 08826, Korea.
Kunsoo RheeDepartment of Biological Sciences, Seoul National University, Seoul 08826, Korea.ORCID 0000-0002-5051-876X

Funding

National Research Foundation of Korea RS-2023-00211612National Research Foundation of Korea RS-2024-00344272Seoul National University
6 · The paper itself

Abstract

The microtubule-organizing activity of centrosomes fluctuates during the cell cycle, reaching the highest levels at M phase. CEP215 (also known as CDK5RAP2) is a key pericentriolar material (PCM) protein for microtubule organization of the human centrosome. Here, we provide evidence that CEP215 exhibits a dynamically suppressed, solid-like state in interphase centrosomes, and becomes a more dynamic state in mitotic centrosomes. Specific interaction with PCNT, another centrosome protein, is crucial for diffusible molecular dynamicity of the CEP215 protein. We also found that the cluster formation activity of CEP215 is impaired in a light-inducible system when its coiled-coil domains (CCDs) are truncated. Defects in spindle pole assembly and spindle formation were accompanied in the cells whose CEP215 is replaced with the CCD-truncated mutants. Our results support the notion that the diffusible mobility of CEP215 is enhanced by both homotypic and heterotypic interactions among CCDs, especially at mitotic spindle poles. This work highlights that biophysical properties of the PCM proteins at the centrosomes fluctuate during the cell cycle.

Indexed as

Cell Cycle ProteinsIntracellular Signaling Peptides and ProteinsMitosisNerve Tissue ProteinsSpindle ApparatusSpindle PolesCentrosomeHeLa CellsHumansMicrotubulesCDK5RAP2 protein, humanCell Cycle ProteinsIntracellular Signaling Peptides and ProteinsNerve Tissue ProteinsCDK5RAP2CentrosomeCEP215Mitotic spindlePericentriolar materialProtein dynamicsSpindle pole

Identifiers

PMID40270183
PMCPMC12148038

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.