Evidence map›Paper›PMID 40269973›Full record

ArticleJournal of animal science and biotechnology2025

Dietary L-leucine supplementation improves ruminal fermentation parameters and epithelium development in fattening Angus beef cattle.

Jishan An, Yu Ge, Huitian He, Hao Ge, Jing Li, Zhiqing Li, Lei Liu, Zuo Wang, Xinyi Lan, Weijun Shen and 2 more

Abstract read
In one paragraph

Article in Journal of animal science and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jishan An *College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Yu Ge *College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Huitian HeCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Hao GeCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Jing LiCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Zhiqing LiCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Lei LiuYuelushan Laboratory, Changsha, 410128, China.
Zuo WangCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Xinyi LanCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Weijun ShenCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Anwei ChengYuelushan Laboratory, Changsha, 410128, China. anweich@126.com.
Fachun WanCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China. wanfc@sina.com.ORCID http://orcid.org/0000-0001-8051-3663

Funding

Key Research and Development Program of Ningxia 2024BBF01008National Key R&D Program 2022YFD1301101-1National Natural Science Foundation of China 32172758
6 · The paper itself

Abstract

backgroundIn this study, the effects of L-leucine (Leu) on rumen fermentation parameters, rumen epithelium development, amino acid composition, rumen bacterial communities and rumen metabolites in beef cattle were investigated. Twenty-four fattening Angus females of similar initial weight (575.5 ± 22.1 kg) were randomly assigned to 2 treatments with 4 replicate pens (3 cattle per pen). They were fed either a basal diet or a basal diet supplemented with 6.0 g L-Leu/100 kg BW/d for 120 d.

results(1) Leu increased the ruminal concentrations of total volatile fatty acid (VFA) (P = 0.017), propionate (P = 0.023), isovalerate (P = 0.001), and branched-chain volatile fatty acid (BCVFA) (P = 0.01) at 4 h post-feeding. It also tended to increase acetate (P = 0.083) and decrease the ammonia-N (NH

conclusionsDietary supplementation with L-Leu altered rumen fermentation parameters and patterns, improved rumen epithelial morphology, and enhanced the expression of genes related to VFA absorption and metabolism in the rumen epithelium of beef cattle.

Indexed as

Beef cattleLeucineRumen epithelial morphologyRumen fermentationRuminal microbiome and metabolome

Identifiers

PMID40269973
PMCPMC12020287

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.