Evidence map›Paper›PMID 40269717›Full record

ArticleBiological procedures online2025

Negative Regulation of Cell Adhesion as a Driver of Brain Metastasis in NSCLC Patients with EGFR Amplification.

Hainan Yang, Congli Hu, Yu Zhou, Taoyuan Tong, Luyao Qi, Weifang Yuan, Changguo Shan, Weiping Hong, Lei Wen, Caicun Zhou and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biological procedures online, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Hainan Yang *Department of Critical Care Medicine, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, 358 Datong Road, Pudong New District, Shanghai, 200137, China.
Congli Hu *Department of Oncology, School of Medicine, Shanghai Pulmonary Hospital & Thoracic Cancer Institute, Tongji University, Shanghai, 200092, China.
Yu Zhou *Department of Medical Genetics, School of Medicine, Tongji University, Shanghai, 200092, China.
Taoyuan TongSchool of Life Sciences and Technology, Tongji University, Shanghai, 200092, China.
Luyao QiDepartment of Critical Care Medicine, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, 358 Datong Road, Pudong New District, Shanghai, 200137, China.
Weifang YuanDepartment of Critical Care Medicine, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, 358 Datong Road, Pudong New District, Shanghai, 200137, China.
Changguo ShanDepartment of Oncology, Guangdong Sanjiu Brain Hospital, Guangzhou, China.
Weiping HongDepartment of Oncology, Guangdong Sanjiu Brain Hospital, Guangzhou, China.
Lei WenDepartment of Radiation Oncology, Zhujiang Hospital, Southern Medical University, 253 Gongye Dadao, Guangzhou, Guangdong, 510280, China. wenlei1998@sina.com.
Caicun ZhouDepartment of Oncology, School of Medicine, Shanghai Pulmonary Hospital & Thoracic Cancer Institute, Tongji University, Shanghai, 200092, China. dr_caicunzhou@126.com.
Ming LeiDepartment of Critical Care Medicine, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, 358 Datong Road, Pudong New District, Shanghai, 200137, China. leiming6891@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Brain metastases are strongly associated with a poor prognosis. Experimental animal models have provided valuable insights into the complex biology underlying brain metastasis, and translating these findings could pave the way for innovative management strategies for patients with brain metastases. Between May 2019 and June 2023, twenty-four lung cancer patients and thirty patients with brain metastases from lung cancer were enrolled at Guangdong Sanjiu Brain Hospital. Next-generation targeted panel sequencing (NGS) was performed on lung cancer tissue and surgical specimens from brain tumors for each patient. Brain metastasis mouse models were established through intracardiac injections, and the brain metastasis rate was analyzed. Our results showed that the rate of EGFR amplification was significantly higher in patients with brain metastases compared to lung cancer patients (40% vs. 12%). EGFR-overexpressing PC9 cell lines demonstrated significantly enhanced proliferation and infiltration abilities compared to their parental PC9 counterparts, as evidenced by CCK-8, wound healing, and transwell assays. Moreover, we observed a much higher brain metastasis rate in mice injected with EGFR-overexpressing PC9 cells compared to those injected with parental PC9 cells. RNA sequencing and Gene Ontology (GO) analysis revealed that differentially expressed genes were primarily associated with the "negative regulation of cell adhesion" in biological processes (BP) and "collagen-containing extracellular matrix" in cellular components (CC). This study identifies the negative regulation of cell adhesion as a key driver of brain metastasis in NSCLC patients with EGFR amplification.

Indexed as

Brain metastasesCell adhesionEGFR amplificationGenotypingNon-small Cell Lung cancer

Identifiers

PMID40269717
PMCPMC12016210

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.