Evidence map›Paper›PMID 40269501›Full record

ArticlePlant communications2025

The Fie1-PRC2 complex regulates H3K27me3 deposition to balance endosperm filling and development in cereals.

Jiechen Wang, Shuai Li, Liujie Wu, Dongsheng Shi, Lina Xu, Zhiping Zhang, Yongyan Wang, Chen Ji, Yuqi Chen, Xueling Zhou and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Jiechen WangState Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology & Ecology, Shanghai 200032, China.
Shuai LiShanghai Oebiotech Co., Ltd., Shanghai 200032, China.
Liujie WuCollege of Life Science, Shanghai Normal University, 100 Guilin Road, Shanghai 200233, China.
Dongsheng ShiNational Key Laboratory of Wheat and Maize Crops Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
Lina XuState Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology & Ecology, Shanghai 200032, China.
Zhiping ZhangState Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology & Ecology, Shanghai 200032, China.
Yongyan WangNational Key Laboratory of Wheat and Maize Crops Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
Chen JiState Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology & Ecology, Shanghai 200032, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
Yuqi ChenState Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology & Ecology, Shanghai 200032, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
Xueling ZhouCollege of Life Science, Shanghai Normal University, 100 Guilin Road, Shanghai 200233, China.
Feifan ZhangCollege of Life Science, Shanghai Normal University, 100 Guilin Road, Shanghai 200233, China.
Mengyao LiState Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.
Xiaohan LiState Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.
Canghao DuThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China.
Qiong WangState Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology & Ecology, Shanghai 200032, China.
Xiaoduo LuInstitute of Molecular Breeding for Maize, Qilu Normal University, Jinan, China.
Wenqin WangCollege of Life Science, Shanghai Normal University, 100 Guilin Road, Shanghai 200233, China.
Guifeng WangNational Key Laboratory of Wheat and Maize Crops Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
Yongrui WuState Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology & Ecology, Shanghai 200032, China; Shanghai Academy of Natural Sciences, Shanghai 200031, China. Electronic address: yrwu@cemps.ac.cn.

Funding

Non-US Government Research Support type
6 · The paper itself

Abstract

Polycomb repressive complex 2 (PRC2)-mediated H3K27me3 deposition is vital for cell fate determination during Arabidopsis thaliana endosperm development. Unlike the transient endosperm in Arabidopsis, cereals follow a different developmental program after the cellularization phase, producing a persistent endosperm. In contrast to the single, constitutively expressed Fertilization Independent Endosperm (FIE) gene in Arabidopsis, cereals have evolved a duplicated, grain-specific counterpart, such as maize ZmFie1 and rice OsFie1. However, their functions remain unclear. We applied Cleavage Under Targets and Tagmentation (CUT&Tag) to profile the dynamics of the H3K27me3 mark during maize endosperm development. We observed a genome-wide elevation of H3K27me3 levels at early stages, followed by a rapid reduction after seed filling. We identified common regions and designated them as Filling Specific Peaks (FSPs), which are largely regulated by ZmFie1. Indeed, knockout of ZmFie1 results in earlier cellularization and slightly enhanced mitosis during endosperm filling, leading to smaller kernels that accumulate more zeins. Consistently, H3K27me3 levels on α-zein genes, located as tandem repeats on chromosome 4, are dramatically decreased in zmfie1. Moreover, it indirectly inhibits cell proliferation by mediating H3K27me3 deposition at ZmMADS loci, thereby balancing endosperm development and filling. Intriguingly, OsFie1 imposes H3K27me3 modification at the loci of 13-kDa prolamin genes and OsMADSs, leading to their repressed expression. Collectively, our findings reveal the conserved function of H3K27me3 deposition mediated by ZmFie1/OsFie1 in cereal endosperm development. The newly evolved, cereal grain-specific FIE1-PRC2 complex plays a key role in balancing storage substance synthesis and cell proliferation during persistent endosperm development.

Indexed as

Edible GrainEndospermHistonesOryzaPlant ProteinsPolycomb Repressive Complex 2Zea maysGene Expression Regulation, PlantHistonesPlant ProteinsPolycomb Repressive Complex 2cell proliferationendosperm fillingH3K27me3maizeZmFie1ZmPRC2

Identifiers

PMID40269501
PMCPMC12177492

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.