Evidence map›Paper›PMID 40269463›Full record

ArticleMicrobiology and immunology2025

Coculture of Bifidobacterium bifidum G9-1 With Butyrate-Producing Bacteria Promotes Butyrate Production.

Haruka Yokota, Yoshiki Tanaka, Hiroshi Ohno

Abstract read
In one paragraph

Article in Microbiology and immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Trial
  2. Article
  3. Biomolecules · 2026
    Article
  4. Article
  5. Impact of lactic acid bacteria andVeterinary and animal science · 2026
    Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Haruka YokotaBiofermin Pharmaceutical Co. Ltd., Kobe, Japan.ORCID http://orcid.org/0009-0009-3709-426X
Yoshiki TanakaBiofermin Pharmaceutical Co. Ltd., Kobe, Japan.
Hiroshi OhnoBiofermin Pharmaceutical Co. Ltd., Kobe, Japan.

Funding

The authors received no specific funding for this work.
6 · The paper itself

Abstract

Supplementation with Bifidobacterium bifidum G9-1 (BBG9-1) has been established to enhance the production of butyrate, a short-chain fatty acid (SCFA) known for its beneficial effects in alleviating constipation. We hypothesized that BBG9-1 alters gut microbiota such that bacteria that produce butyric acid from lactate and acetate become more abundant. In this study, we sought to determine whether BBG9-1 promotes the growth of butyrate-producing bacteria and thereby enhances butyrate production. BBG9-1 was cocultured with different butyrate-producing bacteria to compare differences in the SCFA production of cocultures and monocultures. We indeed detected significant increases in the production of SCFAs in cocultures compared to monocultures. Moreover, lactate and butyrate production increased in a time-dependent manner in the BBG9-1 and Faecalibacterium prausnitzii ID 6052 coculture. In addition, acetate production in cocultures initially increased until 16 h, followed by a decline between 20 and 24 h, and a subsequent significant increase at 48 h. Comparatively, lactate and acetate production in the BBG9-1 and Anaerostipes caccae JCM 13470

Indexed as

BacteriaBifidobacterium bifidumButyratesAcetatesButyric AcidCoculture TechniquesFaecalibacterium prausnitziiFatty Acids, VolatileGastrointestinal MicrobiomeHumansLactic AcidProbioticsAcetatesButyratesButyric AcidFatty Acids, VolatileLactic AcidacetateBifidobacterium bifidum G9‐1 (BBG9‐1)butyrate‐producing bacteriacoculturelactateprobioticsshort‐chain fatty acids (SCFA)

Identifiers

PMID40269463
PMCPMC12232074

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.