Evidence map›Paper›PMID 40269194›Full record

ArticleScientific reports2025

Effect of electromagnetic field radiation on transcriptomic profile and DNA methylation level in pig conceptuses during the peri-implantation period.

Anita Franczak, Agata Zmijewska, Ewa Monika Drzewiecka, Wiktoria Kozlowska, Pawel Wydorski, Lukasz Paukszto, Tiziana L Brevini

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Anita Franczak *Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str. 1A, 10-719, Olsztyn, Poland. anitaf@uwm.edu.pl.ORCID http://orcid.org/0000-0003-2418-8207
Agata Zmijewska *Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str. 1A, 10-719, Olsztyn, Poland. agata.zmijewska@uwm.edu.pl.ORCID http://orcid.org/0000-0002-0661-130X
Ewa Monika DrzewieckaDepartment of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, Tuwima 10, 10-748, Olsztyn, Poland.ORCID http://orcid.org/0000-0002-1328-0190
Wiktoria KozlowskaDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str. 1A, 10-719, Olsztyn, Poland.ORCID http://orcid.org/0000-0002-0260-5199
Pawel WydorskiDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str. 1A, 10-719, Olsztyn, Poland.ORCID http://orcid.org/0000-0002-3098-0199
Lukasz PauksztoDepartment of Botany and Nature Protection, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-719, Olsztyn, Poland.ORCID http://orcid.org/0000-0002-3618-1064
Tiziana L BreviniLaboratory of Biomedical Embryology, Department of Veterinary Medicine and Animal Sciences, Center for Stem Cell Research, Università degli Studi di Milano, 20134, Milan, Italy.ORCID http://orcid.org/0000-0002-0428-3621

Funding

Narodowe Centrum Nauki 2017/25/B/NZ9/00090
6 · The paper itself

Abstract

Extremely low-frequency electromagnetic field (ELF-EMF) radiation alters the steroidogenic activity of porcine conceptuses during the peri-implantation period. This study investigated whether short exposure (2 h) to ELF-EMF radiation may induce changes in the transcriptomic profile of conceptuses and their DNA methylation levels, with a focus on the promoter regions of differentially expressed genes of interest. Porcine conceptuses were collected on days 15-16 of pregnancy, preincubated, and then exposed in vitro to ELF-EMF at a frequency of 50 Hz. ELF-EMF treatment affected the expression of 21 protein-coding transcripts, including solute carrier family 34 member 1 (SLC34A1), hydroxysteroid 17-beta dehydrogenase 2 (HSD17B2), apolipoprotein M (APOM), regucalcin (RGN), heat shock protein family A (Hsp70) member 6 (HSPA6), fibrinogen beta chain, and fibrinogen gamma chain (FGG). There were evaluated 21 GO annotations for biological process terms, nine GO annotations for cellular component terms and two KEGG pathways. The ELF-EMF exposure increased approximately 16 times genomic DNA methylation in conceptuses. ELF-EMF-induced changes in methylation were observed in the promoter regions of APOM, HSD17B2, FGG, and SLC34A1. The results determined 116 predicted single nucleotide variant substitutions within RNA editing sites. In conclusion, ELF-EMF radiation appears to interact with DNA methylation levels and to affect the expression of genes involved mainly in cellular homeostasis, conceptuses development, and attachment, suggesting the need for further investigation to elucidate better the impact of ELF-EMF exposure on the physiology of early conceptuses.

Indexed as

DNA MethylationElectromagnetic FieldsEmbryo ImplantationTranscriptomeAnimalsFemaleGene Expression ProfilingPregnancyPromoter Regions, GeneticSwineConceptusesDNA methylationExtremely low-frequency electromagnetic fieldNext-generation sequencingPig

Identifiers

PMID40269194
PMCPMC12019412

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.