Evidence map›Paper›PMID 40266120›Full record

ArticleVaccines2025

Modular Combinatorial DNA Assembly of Group B Streptococcus Capsular Polysaccharide Biosynthesis Pathways to Expediate the Production of Novel Glycoconjugate Vaccines.

Mark A Harrison, Elizabeth Atkins, Alexandra Faulds-Pain, John T Heap, Brendan W Wren, Ian J Passmore

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Vaccines, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Mark A HarrisonDepartment of Infection Biology, London School of Hygiene & Tropical Medicine, London WC1E 7HT, UK.
Elizabeth AtkinsDepartment of Infection Biology, London School of Hygiene & Tropical Medicine, London WC1E 7HT, UK.ORCID 0000-0002-1783-6889
Alexandra Faulds-PainSchool of Life Sciences, University of Nottingham, Nottingham NG7 2RD, UK.
John T HeapSchool of Life Sciences, University of Nottingham, Nottingham NG7 2RD, UK.
Brendan W WrenDepartment of Infection Biology, London School of Hygiene & Tropical Medicine, London WC1E 7HT, UK.
Ian J PassmoreDepartment of Infection Biology, London School of Hygiene & Tropical Medicine, London WC1E 7HT, UK.ORCID 0000-0002-4418-6957

Funding

Biotechnology and Biological Sciences Research Council BB/W005816/1Biotechnology and Biological Sciences Research Council BB/Y008472/1Medical Research Council CIC2021Wellcome Trust 214227/Z/18/Z
6 · The paper itself

Abstract

BACKGROUND/

objectives

methodsIn this study, we apply combinatorial hierarchical DNA assembly to the heterologous biosynthesis of GBS III, IV and V CPSs in

resultsWe identified several configurations that successfully biosynthesised the GBS CPSs. Furthermore, we exploited the conserved nature of the GBS CPS biosynthesis loci and the flexibility of modular DNA assembly by constructing hybrid pathways from a minimal pool of glycosyltransferase genes. We show that transferase genes with homologous function can be used interchangeably between pathways, obviating the need to clone a complete locus for each new CPS assembly.

conclusionsIn conclusion, we report the first demonstration of heterologous GBS CPS IV and V biosynthesis in

Indexed as

DNA assemblyglycobiologyglycoconjugatesgroup B Streptococcusstart-stop assemblyvaccines

Identifiers

PMID40266120
PMCPMC11946114

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.