ArticlePhysiologia plantarum
Optimizing algal hydrogen photoproduction: a simplified and efficient protocol for anoxic induction in a semi-autotrophic approach.
Article in Physiologia plantarum. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
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4 authors.
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Abstract
Green microalgae, such as Chlamydomonas reinhardtii, show great potential for producing green hydrogen using only water and sunlight, with no carbon emissions. However, sustainable hydrogen production requires addressing the hydrogenase sensitivity to oxygen and enhancing electron allocation to this enzyme. Previous methods for hydrogen photoproduction rely on a brief nitrogen flushing followed by a dark incubation phase to establish anoxia prior to exposure to high light. In this study, we present a straightforward protocol involving a mixotrophic growth phase followed by a semi-autotrophic hydrogen production phase. During the hydrogen production phase, extended nitrogen flushing induced anoxia in the liquid algae culture, even though oxygen was still present in the headspace. Anoxia was maintained under light at moderate intensity (120 μmol m
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