Manganese deficiency or dietary manganese(III) oxide nanoparticle supplementation: consequences for hematology, and intestinal and brain immunity in rats.

Introduction: The study aimed to determine the effect of manganese (Mn) exclusion from the mineral mixture added to the rat diet and replacing the recommended level of MnCO Methods: The experiment was conducted on twenty-four, Wistar rats divided into 3 equal groups. The control (K) group received a diet containing 65 mg/kg of additional Mn originating from the mineral mixture), group B (negative control) was fed a diet deprived of Mn from the mineral mixture, and group N was fed a diet containing 65 mg/kg Mn from Mn2O3NPs preparation. All rats received the experimental diets for 12 weeks. At the end of the experiment, samples of blood, jejunum, and brain were collected from all rats from each group. Results: Mn exclusion from the rat diet led to anemia, worsened the body's immune response, and caused systemic and local inflammation as indicated e.g. by decreased RBC, HCT, and the level of HGB, and CRP in blood, CRP and IgA in the jejunum, and IgG in the brain as well as an increased level of IL-2, IgG and TNF-α in blood, and IL-6 in jejunum. In turn, replacing the recommended level of MnCO Discussion: The obtained results may suggest that the body has activated adaptive mechanisms that efficiently limit the spread of immune system disorders throughout the body.