Evidence map›Paper›PMID 40261708›Full record

ArticleJCI insight2025

New Pseudomonas infections drive Pf phage transmission in CF airways.

Julie D Pourtois, Naomi L Haddock, Aditi Gupta, Arya Khosravi, Hunter A Martinez, Amelia K Schmidt, Prema S Prakash, Ronit Jain, Piper Fleming, Tony H Chang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JCI insight, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Julie D PourtoisBiology Department, Stanford University, Stanford, California, USA.
Naomi L HaddockDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA.
Aditi GuptaDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA.
Arya KhosraviDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA.
Hunter A MartinezDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA.
Amelia K SchmidtDivision of Biological Sciences, University of Montana, Missoula, Montana, USA.
Prema S PrakashDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA.
Ronit JainBiology Department, Stanford University, Stanford, California, USA.
Piper FlemingOceans Department, Stanford University, Pacific Grove, California, USA.
Tony H ChangBiology Department, Stanford University, Stanford, California, USA.
Carlos MillaCenter for Excellence in Pulmonary Biology, Division of Pulmonary Medicine, Department of Pediatrics, Stanford University, Stanford, California, USA.
Patrick R SecorDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, Montana, USA.
Giulio A De LeoOceans Department, Stanford University, Pacific Grove, California, USA.
Paul L BollykyDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA.
Elizabeth B BurgenerCenter for Excellence in Pulmonary Biology, Division of Pulmonary Medicine, Department of Pediatrics, Stanford University, Stanford, California, USA.

Funding

MOLECULAR &CELLULAR IMMUNOBIOLOGYT32AI007290 · NIAID · STANFORD UNIVERSITY · PI Sean Curtis Bendall, Olivia M Martinez · 1985 to 2026
$27.0M
The role of macrophages in chronic suppurative otitis media associated sensory hearing lossR01DC019965 · NIDCD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Peter Luke Santa Maria · 2022 to 2026
$3.6M
Non-invasive Monitoring for Infections and Rejection after Lung TransplantationR01AI182349 · NIAID · STANFORD UNIVERSITY · PI Paul L Bollyky · 2024 to 2026
$1.8M
Pathogenesis of Pf Bacteriophages in Pseudomonas Cystic Fibrosis lung InfectionsR01HL148184 · NHLBI · STANFORD UNIVERSITY · PI MILLA, CARLOS · 2019 to 2022
$1.7M
Studies on bacteriophages in respiratory diseasesK24AI166718 · NIAID · STANFORD UNIVERSITY · PI Paul L Bollyky · 2022 to 2026
$815k
Bacteriophage as a predictive biomarker in chronic Pseudomonas airway diseaseK23HL169902 · NHLBI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI Elizabeth Bendig Burgener · 2023 to 2026
$796k
NHLBI NIH HHS K23 HL169902NHLBI NIH HHS R01 HL148184NIAID NIH HHS K24 AI166718NIAID NIH HHS R01 AI182349NIAID NIH HHS T32 AI007290NIDCD NIH HHS R01 DC019965
6 · The paper itself

Abstract

Pf bacteriophages, lysogenic viruses that infect Pseudomonas aeruginosa (Pa), are implicated in the pathogenesis of chronic Pa infections; phage-infected (Pf+) strains are known to predominate in people with cystic fibrosis (pwCF) who are older and have more severe disease. However, the transmission patterns of Pf underlying the progressive dominance of Pf+ strains are unclear. In particular, it is unknown whether phage transmission commonly occurs horizontally between bacteria via viral particles within the airway or whether Pf+ bacteria are mostly acquired via de novo Pseudomonas infections. Here, we studied Pa genomic sequences from 3 patient cohorts totaling 662 clinical isolates from 105 pwCF. We identified Pf+ isolates and analyzed transmission patterns of Pf within patients between genetically similar groups of bacteria called "clone types." We found that Pf was predominantly passed down vertically within Pa clone types and rarely via horizontal transfer between clone types within the airway. Conversely, we found extensive evidence of Pa de novo infection by a new, genetically distinct Pf+ Pa. Finally, we observed that clinical isolates showed reduced activity of type IV pili and reduced susceptibility to Pf in vitro. These results cast light on the transmission of virulence-associated phages in the clinical setting.

Indexed as

BacteriophagesCystic FibrosisPseudomonas aeruginosaPseudomonas InfectionsPseudomonas PhagesFemaleHumansMaleBacterial infectionsFibrosisInfectious diseaseMicrobiology

Identifiers

PMID40261708
PMCPMC12220970

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.