Evidence map›Paper›PMID 40261631›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Gelatin Zymography: Detecting Gelatinase Activity as a Partner for Invadopodia-Mediated Invasion by Tumor Cells.

Marija Dinevska, Samuel Widodo, Chantel McEntee, Theo Mantamadiotis, Stanley S Stylli

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Marija DinevskaDepartment of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, Australia.
Samuel WidodoDepartment of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, Australia.
Chantel McEnteeDepartment of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, Australia.
Theo MantamadiotisDepartment of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, Australia.
Stanley S StylliDepartment of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, Australia. sstylli@unimelb.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Zymography is a technique for detecting, identifying, and quantifying enzyme activity through gel electrophoresis. Gelatin zymography is used to detect matrix metalloproteinase (MMP) activity (a class of zinc-dependent endopeptidases) such as MMP-2 and MMP-9. We describe a semiquantitative method, optimized for determining the activity of secreted gelatinases in cell culture conditioned medium via the digestion of gelatin embedded in a polyacrylamide gel. This assay can also be partnered with a quantitative "invadopodia degradation" assay, which detects the degradation of FITC-labelled gelatin by actin-rich membrane protrusions known as invadopodia. These projections are enriched in proteolytic enzymes, including MMP-2 and MMP-9 in glioblastoma cells, and are secreted to modify/degrade ECM proteins and facilitate tumor cell invasion.

Indexed as

Electrophoresis, Polyacrylamide GelEnzyme AssaysGelatinGelatinasesNeoplasmsPodosomesCell Line, TumorCulture Media, ConditionedHumansMatrix Metalloproteinase 2Matrix Metalloproteinase 9Neoplasm InvasivenessCulture Media, ConditionedGelatinGelatinasesMatrix Metalloproteinase 2Matrix Metalloproteinase 9GelatinGlioblastomaGliomaInvadopodiaInvasionMatrix metalloproteinasesZymogramZymography

Identifiers

PMID40261631

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.