Evidence map›Paper›PMID 40261629›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Analysis of Metalloproteinase Secretion in Cancer Cells by Zymography.

Javier de Jesus Vasconcelos-Ulloa, Jesus Omar Medina-Cruz, Octavio Galindo-Hernandez

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Seasonal impact of PMScientific reports · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Javier de Jesus Vasconcelos-Ulloa *Multidisciplinary Laboratory of Metabolic and Cancer Studies, Faculty of Medicine, Autonomous University of Baja California (UABC), Mexicali, Baja California, Mexico.
Jesus Omar Medina-Cruz *Multidisciplinary Laboratory of Metabolic and Cancer Studies, Faculty of Medicine, Autonomous University of Baja California (UABC), Mexicali, Baja California, Mexico.
Octavio Galindo-HernandezMultidisciplinary Laboratory of Metabolic and Cancer Studies, Faculty of Medicine, Autonomous University of Baja California (UABC), Mexicali, Baja California, Mexico. octavio.galindo@uabc.edu.mx.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The zymography assay is a semiquantitative polyacrylamide gel electrophoresis technique that allows the separation of matrix metalloproteinases (MMPs) by molecular weight/charge and their detection through their ability to degrade substrates such as collagen (gelatin). The presence of MMPs can be analyzed in cell lysates and conditioned media, observing degradation bands of the gel copolymerized with gelatin. In addition, the size of the degradation bands present in the gel allows comparing the relative presence of MMPs in the analyzed samples. It is a simple and affordable method for the analysis of processes associated with tumor invasion.

Indexed as

Electrophoresis, Polyacrylamide GelEnzyme AssaysMatrix MetalloproteinasesNeoplasmsCell Line, TumorCollagenCulture Media, ConditionedGelatinHumansCollagenCulture Media, ConditionedGelatinMatrix MetalloproteinasesCancerMDA-MB-231 cellsMMP-2MMP-9PC3 cellsZymography

Identifiers

PMID40261629

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.