Evidence map›Paper›PMID 40261620›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Applying Zymography Methods for the Detection of Enzymes from Fungal Endophytes.

Trung Q Do, Enzo A Palombo, Bita Zaferanloo

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Trung Q DoFaculty of Pharmacy, Dai Nam University, Hanoi, Vietnam.
Enzo A PalomboDepartment of Chemistry and Biotechnology, School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Hawthorn, VIC, Australia.
Bita ZaferanlooDepartment of Chemistry and Biotechnology, School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Hawthorn, VIC, Australia. bzaferanloo@swin.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter presents a method for the production and characterization of enzymes derived from fungal endophytes, which are symbiotic organisms residing within plants. Enzyme production is induced by cultivating the fungi on optimized growth media, and the resulting protein levels are monitored using the Bradford assay over a defined duration. Following protein depletion, the media is purified, and distinct enzymes are characterized using substrate-specific zymography employing sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and appropriate buffers. Our findings underscore the efficacy of zymography in swiftly characterizing enzymes derived from fungal endophytes. This method holds significant promise for various biotechnological and industrial applications including, but not limited to, biofuel production and pharmaceuticals.

Indexed as

Electrophoresis, Polyacrylamide GelEndophytesEnzyme AssaysFungal ProteinsFungiFungal ProteinsBradford assayEndophytesFungal enzymesProtein utilizationZymography

Identifiers

PMID40261620

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.