Evidence map›Paper›PMID 40261617›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Zymography Techniques for the Profiling of Digestive Protease in a Freshwater Invertebrate Model.

Federico A Dellagnola, Martín S Godoy, Israel A Vega

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Federico A DellagnolaInstitute of Histology and Embryology of Mendoza, National Scientific and Technical Research Council and National University of Cuyo (IHEM-CONICET, UNCuyo), Mendoza, Argentina.
Martín S GodoyInstitute of Research in Chemical Sciences, School of Chemical and Technological Sciences, Catholic University of Cuyo (FCQyT, UCCuyo), San Juan, Argentina.
Israel A VegaInstitute of Histology and Embryology of Mendoza, National Scientific and Technical Research Council and National University of Cuyo (IHEM-CONICET, UNCuyo), Mendoza, Argentina. ivega@mendoza-conicet.gob.ar.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Zymography is a sensitive and specific technique that enables the detection and characterization of proteases of low abundance. Here, we describe two zymographic techniques, in-gel and in situ zymography, to discover proteases (20-120 kDa) along the gut of apple snails. Proteases of different molecular weights are separated by electrophoresis in gelatin copolymerized sodium dodecyl sulfate (SDS) polyacrylamide gels and then enzymatic activities revealed by Coomassie Blue negative staining. Protease families can be identified in the presence of specific inhibitors. We also use in situ zymography for localizing proteases in intracellular symbiotic corpuscles that habit in the digestive gland of the gastropod Pomacea canaliculata. Different spatial-temporal scenarios of protease synthesis, secretion, and hydrolysis of dietary proteins may be identified by a combination of in-gel and in situ zymography.

Indexed as

Electrophoresis, Polyacrylamide GelEnzyme AssaysPeptide HydrolasesSnailsAnimalsDigestive SystemFresh WaterPeptide HydrolasesApple snailsGastropodaIn-gel zymographyIn situ zymographyInvasive speciesSerine-protease

Identifiers

PMID40261617

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.