ArticleMethods in molecular biology (Clifton, N.J.)2025
Evaluating Matrix Metalloproteinase Expression in Primary Mouse Glial Cultures Using Gel Zymography.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microglia and astrocytes are major glial cell types in the central nervous system (CNS). Under normal conditions, they play a variety of supportive and protective functions, but when inappropriately stimulated by excessive or chronic stimuli, they can become detrimental to CNS health, both in their own effector functions and by orchestrating inflammatory processes. Microglia and astrocytes both produce matrix metalloproteinases (MMPs), which play important roles in physiological and pathological processes. To characterize MMP expression in glial cells, and to gain insights into the roles they play in regulating tissue repair and remodeling, we employ the method of gel zymography to quantify levels of MMP-2 and MMP-9, as well as their inactive pro-forms. This technique is relatively easy and simple to perform and provides quick and quantifiable data. In this chapter, we describe this approach to analyze MMP expression in supernatants from primary mouse cultures of microglia and astrocytes.
Indexed as
Identifiers
40261615What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.