Evidence map›Paper›PMID 40261045›Full record

ArticlemSphere2025

TEAL-Seq: targeted expression analysis sequencing.

Georgia Doing, Priya Shanbhag, Isaac Bell, Sara Cassidy, Efthymios Motakis, Elizabeth Aiken, Julia Oh, Mark D Adams

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mSphere, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Georgia DoingThe Jackson Laboratory for Genomic Medicine Farmington, Farmington, Connecticut, USA.ORCID 0000-0002-0835-6955
Priya ShanbhagThe Jackson Laboratory for Genomic Medicine Farmington, Farmington, Connecticut, USA.
Isaac BellThe Jackson Laboratory for Genomic Medicine Farmington, Farmington, Connecticut, USA.
Sara CassidyThe Jackson Laboratory for Genomic Medicine Farmington, Farmington, Connecticut, USA.
Efthymios MotakisThe Jackson Laboratory for Genomic Medicine Farmington, Farmington, Connecticut, USA.
Elizabeth AikenThe Jackson Laboratory for Genomic Medicine Farmington, Farmington, Connecticut, USA.
Julia OhThe Jackson Laboratory for Genomic Medicine Farmington, Farmington, Connecticut, USA.
Mark D AdamsThe Jackson Laboratory for Genomic Medicine Farmington, Farmington, Connecticut, USA.ORCID 0000-0003-3161-7460

Funding

Transcriptomics compendia for the study of strain-level genetic diversity of the human skin microbiomeF32GM151856 · NIGMS · JACKSON LABORATORY · PI DOING, GEORGIA · 2023 to 2024
$143k
NIAMS NIH HHS L70 AR084912NIGMS NIH HHS F32 GM151856
6 · The paper itself

Abstract

Metagenome sequencing enables the genetic characterization of complex microbial communities. However, determining the activity of isolates within a community presents several challenges, including the wide range of organismal and gene expression abundances, the presence of host RNA, and low microbial biomass at many sites. To address these limitations, we developed "targeted expression analysis sequencing" or TEAL-seq, enabling sensitive species-specific analyses of gene expression using highly multiplexed custom probe pools. For proof of concept, we targeted about 1,700 core and accessory genes of

Indexed as

Gene Expression ProfilingMetagenomicsMicrobiotaStaphylococcus aureusStaphylococcus epidermidisHigh-Throughput Nucleotide SequencingHumansMetagenomeSkinmetagenomicsskin microbiometargeted sequencing

Identifiers

PMID40261045
PMCPMC12108068

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.