Evidence map›Paper›PMID 40259360›Full record

ArticleBMC research notes2025

In vitro RNA-mediated gene silencing of Fusarium oxysporum f.sp. cubense from Ecuador and assessment of RNAi molecule stability in banana plants.

Ricardo Pacheco, Julio Bonilla, Aracely Paguay, Freddy Magdama, Pablo Chong

Abstract read
In one paragraph

Article in BMC research notes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ricardo PachecoESPOL, Centro de Investigaciones Biotecnológicas del Ecuador, ESPOL Polytechnic University, Campus Gustavo Galindo, Km 30.5 Vía Perimetral, Guayaquil, 090902, Ecuador.
Julio BonillaESPOL, Centro de Investigaciones Biotecnológicas del Ecuador, ESPOL Polytechnic University, Campus Gustavo Galindo, Km 30.5 Vía Perimetral, Guayaquil, 090902, Ecuador.
Aracely PaguayESPOL, Centro de Investigaciones Biotecnológicas del Ecuador, ESPOL Polytechnic University, Campus Gustavo Galindo, Km 30.5 Vía Perimetral, Guayaquil, 090902, Ecuador.
Freddy MagdamaESPOL, Centro de Investigaciones Biotecnológicas del Ecuador, ESPOL Polytechnic University, Campus Gustavo Galindo, Km 30.5 Vía Perimetral, Guayaquil, 090902, Ecuador.
Pablo ChongESPOL, Centro de Investigaciones Biotecnológicas del Ecuador, ESPOL Polytechnic University, Campus Gustavo Galindo, Km 30.5 Vía Perimetral, Guayaquil, 090902, Ecuador. pachong@espol.edu.ec.

Funding

Escuela Superior Politécnica del Litoral CIBE-24-2021
6 · The paper itself

Abstract

objectiveFusarium wilt poses a significant threat to banana production, caused by diverse clonal Fusarium lineages. Given the lack of curative measures, developing effective treatments is crucial. RNA interference (RNAi) technology, utilizing double-stranded RNA (dsRNA) molecules, offers a promising solution. In this study, RNAi was evaluated by silencing the activity of the Beta-tubulin (Focβ-tub), C5 Sterol desaturase (FocERG3) and Chitin synthase 1 (FocChs1) genes in a pathogenic Fusarium strain. Furthermore, we study the potential of dsRNA translocation in 3 months old banana plants at early hours of been spray under greenhouse conditions.

resultsIn vitro results demonstrated that dsRNA-FocChs1 was more effective in inhibiting spores, with an average IC

Indexed as

FusariumMusaPlant DiseasesRNA, Double-StrandedRNA InterferenceChitin SynthaseTubulinChitin SynthaseRNA, Double-StrandedTubulinBananaFusarium oxysporum F.sp. CubenseRNA interference

Identifiers

PMID40259360
PMCPMC12013083

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.