Evidence map›Paper›PMID 40259189›Full record

ArticleImmunologic research2025

The urease E subunit vaccine stimulate the immune response versus Helicobacter pylori in animal model.

Maedeh Nikzad-Chaleshtori, Mohsen Asgari, Golnoosh Rezaeizadeh, Faranak Aali, Abbas Doosti

Abstract read
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In one paragraph

Article in Immunologic research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. UnravelingVaccines · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Maedeh Nikzad-ChaleshtoriDepartment of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Mohsen AsgariBiotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Golnoosh RezaeizadehDepartment of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Faranak AaliBiotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Abbas DoostiBiotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. doostiabbas2021@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There is a strong association between Helicobacter pylori (H. pylori) and the occurrence of gastritis and gastric mucosal lymphoma in the human population. Vaccination is a viable preventive measure in light of the escalating issue of antibiotic resistance. The use of DNA vaccines presents a potentially effective approach. This study used the utilization of antigenic H. pylori urease E subunit (UreE) for the development of a DNA vaccine. The UreE gene was chemically cloned into pIRES2-DsRed-Express (pDNA), and PCR and restriction enzyme digestion verified the cloning. The immunogenicity and immune-protective efficacy of the vaccination were assessed in BALB/c mice. In contrast, blood samples from BALB/c mice inoculated with pDNA-UreE showed higher levels of IgG, IFN-γ, IL- 4, and IL- 17. Furthermore, stomach damage and bacterial loads were reduced, and BALB/c mice inoculated with pDNA-UreE exhibited a significant protection rate (87.5%) against the H. pylori challenge. pDNA-UreE generated a combination of Th1-Th2-Th17 immune responses, perhaps contributing to adequate protection. Based on our findings, using this DNA immunization as a preventive measure against H. pylori infection is a viable approach.

Indexed as

Bacterial ProteinsBacterial VaccinesHelicobacter InfectionsHelicobacter pyloriUreaseVaccines, DNAAnimalsAntibodies, BacterialCytokinesDisease Models, AnimalFemaleHumansImmunoglobulin GMiceMice, Inbred BALB CVaccines, SubunitAntibodies, BacterialBacterial ProteinsBacterial VaccinesCytokinesImmunoglobulin GUreaseVaccines, DNAVaccines, SubunitDNA vaccineHelicobacter pyloriUrease E subunit

Identifiers

PMID40259189

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.