Evidence map›Paper›PMID 40259087›Full record

ArticleBiotechnology letters2025

Design and application of expression constructs for FMDV serotype O structural proteins.

Mostafa R Zaher, Dalia M El-Husseini, Mohamed H El-Husseiny, Azza M El Amir, Naglaa M Hagag, Reham H Tammam

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Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Mostafa R ZaherGenome Research Unit, Animal Health Research Institute, Agriculture Research Center (ARC), Giza, 12618, Egypt. mostafa.r.zaher@ahri.gov.eg.ORCID http://orcid.org/0000-0002-8267-2702
Dalia M El-HusseiniNanomaterials Research and Synthesis Unit, Animal Health Research Institute, Agriculture Research Center (ARC), Doka, Giza, 12618, Egypt.
Mohamed H El-HusseinyReference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center (ARC), Giza, 12618, Egypt.
Azza M El AmirBiotechnology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt.
Naglaa M HagagGenome Research Unit, Animal Health Research Institute, Agriculture Research Center (ARC), Giza, 12618, Egypt.
Reham H TammamChemistry Department, Faculty of Science, Cairo University, Giza, 12613, Egypt. reham_tammam@cu.edu.eg.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Design and validate flexible constructs for recombinant expression of FMDV serotype O structural proteins of the circulating topotypes using newly designed degenerate primers. The designed degenerate primers targeting diverse topotypes enabled the successful amplification of VP0, VP1, and VP3 genes. Integration of the essential transcriptional and translational regulatory elements including T7 promoter, leader g10 sequence, and T7 terminator, as well as ribosome binding site (RBS), start and stop codons, respectively via overlap extension PCR empowered efficient expression of these proteins in E. coli. Cloned constructs expressed the target proteins of expected molecular weights: VP0 (34 kDa), VP1 (24 kDa), and VP3 (22 kDa). SDS-PAGE and Western blotting confirmed high protein yield and purity. This platform demonstrated adaptability for diagnostic and vaccine development applications. The workflow offers a robust tool for producing FMDV structural proteins concerning the circulating strains attempting to improve control measures including diagnosis and vaccinations.

Indexed as

Foot-and-Mouth Disease VirusViral Structural ProteinsCloning, MolecularEscherichia coliRecombinant ProteinsSerogroupRecombinant ProteinsViral Structural ProteinsDiagnosisFoot-and-mouth disease virus (FMDV)Protein productionStructural proteinsVaccine

Identifiers

PMID40259087

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.