ArticleBiodegradation2025
Development of a robust enzyme cascade system: co-immobilization of laccase and versatile peroxidase on polyacrylamide hydrogel for enhanced BPA degradation.
Article in Biodegradation, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Immobilization Agaricus bisporus laccase via covalent attachment and metal chelate interaction: removal of reactive brown-10 dye.Biodegradation · 2026Article
- Synthetic Biology Approaches to Enzymology in Food and Agriculture Systems.Journal of agricultural and food chemistry · 2026Review
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Authors and funding
5 authors.
Funding
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Abstract
Biodegradation using a synergically integrated system of laccase (E.C. 1.10.3.2) and versatile peroxidase (EC 1.11.1.16) co-immobilized on the polyacrylamide (PAM) hydrogel presents a promising solution for removing endocrine disrupting chemicals (EDCs) like bisphenol A (BPA) from wastewater. In this study, we developed a tailored biocatalyst consisting of a fungal laccase from Pleurotus ostreatus IBL-02 and versatile peroxidase, enzyme cascade co-immobilized covalently on a 7% (w/v) PAM hydrogel, offering high catalytic potential across various pH and temperature ranges. The PAM-VP/Lac structure was analyzed using scanning electron microscopy and Fourier-transform infrared spectrophotometry, revealing improved characteristics compared to free counterparts (FLac and FVP). The optimal pH for FLac, FVP, Lac/VP, and PAM-VP/Lac was 4, 5, 6, and 7, respectively. PAM-VP/Lac exhibited optimal activity at 50-60 °C, higher than FLac, FVP, and Lac-VP. PAM-VP/Lac showed superior operational stability, retaining 99.2% of its activity after eight cycles, with an immobilization efficiency of 78.62 ± 1.15% and activity recovery of 33.71 ± 0.2%. It also demonstrated enhanced thermal stability, with a two-fold increase in half-life at 50-70 °C. Thermodynamic analysis showed significant improvements in stability parameters for PAM-VP/Lac. This system achieved complete BPA degradation within two and a half hr, highlighting its potential for industrial-scale environmental remediation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.